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Journal of Bacteriology, January 2004, p. 575-579, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.575-579.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

PmrAB, a Two-Component Regulatory System of Pseudomonas aeruginosa That Modulates Resistance to Cationic Antimicrobial Peptides and Addition of Aminoarabinose to Lipid A

Samuel M. Moskowitz,1 Robert K. Ernst,2 and Samuel I. Miller2,3,4*

Departments of Pediatrics,1 Medicine,2 Microbiology,3 Genome Sciences, University of Washington School of Medicine, Seattle, Washington 981954

Received 27 June 2003/ Accepted 19 October 2003

Spontaneous polymyxin-resistant mutants of Pseudomonas aeruginosa were isolated. The mutations responsible for this phenotype were mapped to a two-component signal transduction system similar to PmrAB of Salmonella enterica serovar Typhimurium. Lipid A of these mutants contained aminoarabinose, an inducible modification that is associated with polymyxin resistance. Thus, P. aeruginosa possesses a mechanism that induces resistance to cationic antimicrobial peptides in response to environmental conditions.

* Corresponding author. Mailing address: Departments of Medicine, Microbiology, and Genome Sciences, University of Washington School of Medicine, Room K140 HSB, Box 357710, Seattle, WA 98195-7710. Phone: (206) 616-5107. Fax: (206) 616-4295. E-mail: millersi{at}u.washington.edu.

Journal of Bacteriology, January 2004, p. 575-579, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.575-579.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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