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Journal of Bacteriology, January 2004, p. 588-592, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.588-592.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Biphasic Excitation by Leucine in Escherichia coli Chemotaxis

Shahid Khan1* and David R. Trentham2

Molecular Biology Consortium, Chicago, Illinois 60612,1 National Institute for Medical Research, London NW7 1AA, United Kingdom2

Received 29 July 2003/ Accepted 10 October 2003

Leucine concentration jumps (applied by photolysis of inert derivatives) triggered swim or tumble responses in Escherichia coli mutants lacking Tsr or Tar, respectively. Wild-type E. coli bacteria were attracted in spatial assays when the initial leucine concentration difference was 5 to 120 µM but were repulsed when it was over 0.5 mM. Their responses to concentration jumps confirmed earlier deductions regarding biphasic excitation.


* Corresponding author. Mailing address: Molecular Biology Consortium, Chicago Technology Park, 2201 W. Campbell Park Dr., Chicago, IL 60612. Phone: (312) 942-9017. Fax: (312) 829-4069. E-mail: kh01{at}tigger.uic.edu.


Journal of Bacteriology, January 2004, p. 588-592, Vol. 186, No. 2
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.2.588-592.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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