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Journal of Bacteriology, November 2004, p. 7804-7806, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7804-7806.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of an Antisense RNA Strategy To Investigate the Functional Significance of Mn-Catalase in the Extreme Thermophile Thermus thermophilus

Renata Moreno,1 Aurelio Hidalgo,2 Felipe Cava,1 Roberto Fernández-Lafuente,2 José Manuel Guisán,2 and José Berenguer1*

Centro de Biología Molecular "Severo Ochoa" CSIC-UAM,1 Departamento de Biocatálisis, Instituto de Catálisis y Petroleoquímica-CSIC, Campus de Cantoblanco, Madrid, Spain2

Received 27 May 2004/ Accepted 15 August 2004

The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the antisense RNA against the selected target.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa" CSIC-UAM, Campus de Cantoblanco, 28049 Madrid, Spain. Phone: 34914978099. Fax: 34914978087. E-mail: jberenguer{at}cbm.uam.es.

Journal of Bacteriology, November 2004, p. 7804-7806, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7804-7806.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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