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Journal of Bacteriology, November 2004, p. 7807-7810, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7807-7810.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Long-Term Effect of Mutagenic DNA Repair on Accumulation of Mutations in Pseudomonas syringae B86-17

Shouan Zhang1 and George W. Sundin2,3,4*

National Center for Agricultural Utilization Research, USDA Agricultural Research Service, Peoria, Illinois,1 Program in Genetics,2 Center for Microbial Ecology,3 Department of Plant Pathology, Michigan State University, East Lansing, Michigan4

Received 15 July 2004/ Accepted 12 August 2004

Forty replicate lineages of Pseudomonas syringae B86-17 cells expressing the rulAB mutagenic DNA repair (MDR) determinant or the rulB::Km MDR-deficient mutant GWS242 were passaged through single-cell bottlenecks (60 cycles), with a UV radiation (UVR) exposure given to half of the lineages at the beginning of each cycle. After every 10th bottleneck cycle, single-colony isolates from all 80 lineages were subjected to 39 phenotypic screens, with newly arising mutations detected in 60 and 0% of UVR-exposed or non-UVR-exposed B86-17 lineages, respectively, by the 60th cycle. Cellular fitness, measured as growth rate in a minimal medium, of UVR-exposed lineages of both B86-17 and GWS242 after 60 cycles was not significantly different from that of the ancestral strains. Although UVR exposure and MDR activity increased the occurrence of mutations in cells, a significant reduction in overall fitness was not observed.

* Corresponding author. Mailing address: Michigan State University, 103 Center for Integrated Plant Systems, East Lansing, MI 48824-1311. Phone: (517) 355-4573. Fax: (517) 353-5598. E-mail: sundin{at}msu.edu.

Journal of Bacteriology, November 2004, p. 7807-7810, Vol. 186, No. 22
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.22.7807-7810.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Weigand, M. R., Sundin, G. W. (2009). Long-Term Effects of Inducible Mutagenic DNA Repair on Relative Fitness and Phenotypic Diversification in Pseudomonas cichorii 302959. Genetics 181: 199-208 [Abstract] [Full Text]  


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