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Journal of Bacteriology, November 2004, p. 7815-7817, Vol. 186, No. 22
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.22.7815-7817.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Cuprous Oxidase Activity of CueO from Escherichia coli
Satish K. Singh,1
Gregor Grass,2
Christopher Rensing,3 and
William R. Montfort1*
Departments of Biochemistry and Molecular Biophysics,1
Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona,3
Institute for Microbiology, Martin Luther University, Halle, Germany2
Received 17 June 2004/
Accepted 16 August 2004
We have found CueO from Escherichia coli to have a robust cuprous oxidase activity, severalfold higher than any homologue. These data suggest that a functional role for CueO in protecting against copper toxicity in vivo includes the removal of Cu(I).
* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biophysics, BSW 518, University of Arizona, Tucson, AZ 85721. Phone: (520) 621-1884. Fax: (520) 621-1697. E-mail:
montfort{at}email.arizona.edu.
Journal of Bacteriology, November 2004, p. 7815-7817, Vol. 186, No. 22
0021-9193/04/$08.00+0 DOI: 10.1128/JB.186.22.7815-7817.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
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