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Journal of Bacteriology, December 2004, p. 8156-8158, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.8156-8158.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803

Sam J. Barkley, Shrivallabh B. Desai, and C. Dale Poulter*

Department of Chemistry, University of Utah, Salt Lake City, Utah

Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His6-tagged protein was produced in Escherichia coli and purified by Ni2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg2+ for activity; KmIPP was 52 µM, and kcatIPP was 0.23 s–1.

* Corresponding author. Mailing address: 315 South 1400 East RM 2020, Department of Chemistry, University of Utah, Salt Lake City, UT 84112. Phone: (801) 581-6685. Fax: (801) 581-4391. E-mail: poulter{at}chem.utah.edu.

Journal of Bacteriology, December 2004, p. 8156-8158, Vol. 186, No. 23
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.23.8156-8158.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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