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Journal of Bacteriology, December 2004, p. 8533-8536, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8533-8536.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Interaction between the TolC and AcrA Proteins of a Multidrug Efflux System of Escherichia coli

Fasahath Husain,1,2 Matthew Humbard,2 and Rajeev Misra2*

Microbiology Graduate Program,1 School of Life Sciences, Arizona State University, Tempe, Arizona2

Received 24 May 2004/ Accepted 6 September 2004

This paper provides the biochemical evidence for physical interactions between the outer membrane component, TolC, and the membrane fusion protein component, AcrA, of the major antibiotic efflux pump of Escherichia coli. Cross-linking between TolC and AcrA was independent of the presence of any externally added substrate of the efflux pump or of the pump protein, AcrB. The biochemical demonstration of a TolC-AcrA interaction is consistent with genetic studies in which extragenic suppressors of a mutant TolC strain were found in the acrA gene.

* Corresponding author. Mailing address: School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501. Phone: (480) 965-3320. Fax: (480) 965-6899. E-mail: rajeev.misra{at}asu.edu.

Journal of Bacteriology, December 2004, p. 8533-8536, Vol. 186, No. 24
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.24.8533-8536.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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