This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Labbate, M.
Right arrow Articles by Kjelleberg, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Labbate, M.
Right arrow Articles by Kjelleberg, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2004, p. 692-698, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.692-698.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Quorum Sensing-Controlled Biofilm Development in Serratia liquefaciens MG1

Maurizio Labbate,1,2 Shu Yeong Queck,1,2 Kai Shyang Koh,1,2 Scott A. Rice,1,2 Michael Givskov,3 and Staffan Kjelleberg1,2*

School of Biotechnology and Biomolecular Sciences,1 Centre for Marine Biofouling and Bio-Innovation, University of New South Wales, Sydney, New South Wales, Australia,2 Section of Molecular Microbiology, BioCentrum-DTU, The Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark3

Received 27 May 2003/ Accepted 27 October 2003

Serratia liquefaciens MG1 contains an N-acylhomoserine lactone-mediated quorum-sensing system that is known to regulate swarming motility colonization. In this study, we describe for S. liquefaciens MG1 the development of a novel biofilm consisting of cell aggregates and differentiated cell types, such as cell chains and long filamentous cells. Furthermore, quorum sensing is shown to be crucial for normal biofilm development and for elaborate differentiation. A mutant of S. liquefaciens MG1 that was incapable of synthesizing extracellular signal formed a thin and nonmature biofilm lacking cell aggregates and differentiated cell chains. Signal-based complementation of this mutant resulted in a biofilm with the wild-type architecture. Two quorum-sensing-regulated genes (bsmA and bsmB) involved in biofilm development were identified, and we propose that these genes are engaged in fine-tuning the formation of cell aggregates at a specific point in biofilm development.

* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia 2052. Phone: 61-2-9385 2102. Fax: 61-2-9385 1779. E-mail: s.kjelleberg{at}unsw.edu.au.

Journal of Bacteriology, February 2004, p. 692-698, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.692-698.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Khajanchi, B. K., Sha, J., Kozlova, E. V., Erova, T. E., Suarez, G., Sierra, J. C., Popov, V. L., Horneman, A. J., Chopra, A. K. (2009). N-Acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology 155: 3518-3531 [Abstract] [Full Text]  
  • Vejborg, R. M., Klemm, P. (2009). Cellular chain formation in Escherichia coli biofilms. Microbiology 155: 1407-1417 [Abstract] [Full Text]  
  • Janssens, J. C. A., Steenackers, H., Robijns, S., Gellens, E., Levin, J., Zhao, H., Hermans, K., De Coster, D., Verhoeven, T. L., Marchal, K., Vanderleyden, J., De Vos, D. E., De Keersmaecker, S. C. J. (2008). Brominated Furanones Inhibit Biofilm Formation by Salmonella enterica Serovar Typhimurium. Appl. Environ. Microbiol. 74: 6639-6648 [Abstract] [Full Text]  
  • Ryan, R. P., Dow, J. M. (2008). Diffusible signals and interspecies communication in bacteria. Microbiology 154: 1845-1858 [Abstract] [Full Text]  
  • Merritt, P. M., Danhorn, T., Fuqua, C. (2007). Motility and Chemotaxis in Agrobacterium tumefaciens Surface Attachment and Biofilm Formation. J. Bacteriol. 189: 8005-8014 [Abstract] [Full Text]  
  • Shanks, R. M. Q., Stella, N. A., Kalivoda, E. J., Doe, M. R., O'Dee, D. M., Lathrop, K. L., Guo, F. L., Nau, G. J. (2007). A Serratia marcescens OxyR Homolog Mediates Surface Attachment and Biofilm Formation. J. Bacteriol. 189: 7262-7272 [Abstract] [Full Text]  
  • Morohoshi, T., Shiono, T., Takidouchi, K., Kato, M., Kato, N., Kato, J., Ikeda, T. (2007). Inhibition of Quorum Sensing in Serratia marcescens AS-1 by Synthetic Analogs of N-Acylhomoserine Lactone. Appl. Environ. Microbiol. 73: 6339-6344 [Abstract] [Full Text]  
  • Lee, J., Bansal, T., Jayaraman, A., Bentley, W. E., Wood, T. K. (2007). Enterohemorrhagic Escherichia coli Biofilms Are Inhibited by 7-Hydroxyindole and Stimulated by Isatin. Appl. Environ. Microbiol. 73: 4100-4109 [Abstract] [Full Text]  
  • Guan, C., Ju, J., Borlee, B. R., Williamson, L. L., Shen, B., Raffa, K. F., Handelsman, J. (2007). Signal Mimics Derived from a Metagenomic Analysis of the Gypsy Moth Gut Microbiota. Appl. Environ. Microbiol. 73: 3669-3676 [Abstract] [Full Text]  
  • Labbate, M., Zhu, H., Thung, L., Bandara, R., Larsen, M. R., Willcox, M. D. P., Givskov, M., Rice, S. A., Kjelleberg, S. (2007). Quorum-Sensing Regulation of Adhesion in Serratia marcescens MG1 Is Surface Dependent. J. Bacteriol. 189: 2702-2711 [Abstract] [Full Text]  
  • Koh, K. S., Lam, K. W., Alhede, M., Queck, S. Y., Labbate, M., Kjelleberg, S., Rice, S. A. (2007). Phenotypic Diversification and Adaptation of Serratia marcescens MG1 Biofilm-Derived Morphotypes. J. Bacteriol. 189: 119-130 [Abstract] [Full Text]  
  • Gonzalez, J. E., Keshavan, N. D. (2006). Messing with Bacterial Quorum Sensing. Microbiol. Mol. Biol. Rev. 70: 859-875 [Abstract] [Full Text]  
  • Moons, P., Van Houdt, R., Aertsen, A., Vanoirbeek, K., Engelborghs, Y., Michiels, C. W. (2006). Role of Quorum Sensing and Antimicrobial Component Production by Serratia plymuthica in Formation of Biofilms, Including Mixed Biofilms with Escherichia coli. Appl. Environ. Microbiol. 72: 7294-7300 [Abstract] [Full Text]  
  • Coulthurst, S. J., Williamson, N. R., Harris, A. K. P., Spring, D. R., Salmond, G. P. C. (2006). Metabolic and regulatory engineering of Serratia marcescens: mimicking phage-mediated horizontal acquisition of antibiotic biosynthesis and quorum-sensing capacities. Microbiology 152: 1899-1911 [Abstract] [Full Text]  
  • Dubern, J.-F., Lugtenberg, B. J. J., Bloemberg, G. V. (2006). The ppuI-rsaL-ppuR Quorum-Sensing System Regulates Biofilm Formation of Pseudomonas putida PCL1445 by Controlling Biosynthesis of the Cyclic Lipopeptides Putisolvins I and II.. J. Bacteriol. 188: 2898-2906 [Abstract] [Full Text]  
  • Herzberg, M., Kaye, I. K., Peti, W., Wood, T. K. (2006). YdgG (TqsA) Controls Biofilm Formation in Escherichia coli K-12 through Autoinducer 2 Transport. J. Bacteriol. 188: 587-598 [Abstract] [Full Text]  
  • Gonzalez Barrios, A. F., Zuo, R., Hashimoto, Y., Yang, L., Bentley, W. E., Wood, T. K. (2006). Autoinducer 2 Controls Biofilm Formation in Escherichia coli through a Novel Motility Quorum-Sensing Regulator (MqsR, B3022). J. Bacteriol. 188: 305-316 [Abstract] [Full Text]  
  • Farah, C., Vera, M., Morin, D., Haras, D., Jerez, C. A., Guiliani, N. (2005). Evidence for a Functional Quorum-Sensing Type AI-1 System in the Extremophilic Bacterium Acidithiobacillus ferrooxidans. Appl. Environ. Microbiol. 71: 7033-7040 [Abstract] [Full Text]  
  • Tomlin, K. L., Malott, R. J., Ramage, G., Storey, D. G., Sokol, P. A., Ceri, H. (2005). Quorum-Sensing Mutations Affect Attachment and Stability of Burkholderia cenocepacia Biofilms. Appl. Environ. Microbiol. 71: 5208-5218 [Abstract] [Full Text]  
  • Ren, D., Zuo, R., Gonzalez Barrios, A. F., Bedzyk, L. A., Eldridge, G. R., Pasmore, M. E., Wood, T. K. (2005). Differential Gene Expression for Investigation of Escherichia coli Biofilm Inhibition by Plant Extract Ursolic Acid. Appl. Environ. Microbiol. 71: 4022-4034 [Abstract] [Full Text]  
  • Rice, S. A., Koh, K. S., Queck, S. Y., Labbate, M., Lam, K. W., Kjelleberg, S. (2005). Biofilm Formation and Sloughing in Serratia marcescens Are Controlled by Quorum Sensing and Nutrient Cues. J. Bacteriol. 187: 3477-3485 [Abstract] [Full Text]  
  • Parsek, M. R., Fuqua, C. (2004). Biofilms 2003: Emerging Themes and Challenges in Studies of Surface-Associated Microbial Life. J. Bacteriol. 186: 4427-4440 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»