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Journal of Bacteriology, February 2004, p. 777-784, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.777-784.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The RecA Protein of Helicobacter pylori Requires a Posttranslational Modification for Full Activity

Wolfgang Fischer* and Rainer Haas

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, D-80336 Munich, Germany

Received 18 July 2003/ Accepted 27 October 2003

The RecA protein is a central component of the homologous recombination machinery and of the SOS system in most bacteria. In performing these functions, it is involved in DNA repair processes and plays an important role in natural transformation competence. This may be especially important in Helicobacter pylori, where an unusually high degree of microdiversity among strains is generated by homologous recombination. We have suggested previously that the H. pylori RecA protein is subject to posttranslational modifications that result in a slight shift in its electrophoretic mobility. Here we show that at least two genes downstream of recA are involved in this modification and that this process is dependent on genes involved in glycosylation and lipopolysaccharide biosynthesis. Site-directed mutagenesis of a putative glycosylation site results in production of an unmodified RecA protein. This posttranslational modification is not involved in membrane targeting or cell division functions but is necessary for the full function of RecA in DNA repair. Thus, it might be an adaptation to the specific requirements of H. pylori in its natural environment.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: 49-89-51605277. Fax: 49-89-51605223. E-mail: fischer{at}m3401.mpk.med.uni-muenchen.de.

Journal of Bacteriology, February 2004, p. 777-784, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.777-784.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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