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Journal of Bacteriology, February 2004, p. 870-874, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.870-874.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

DNA Binding by the Meningococcal RdgC Protein, Associated with Pilin Antigenic Variation

Timothy Moore, Gary J. Sharples,{dagger} and Robert G. Lloyd*

Institute of Genetics, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, United Kingdom

Received 29 July 2003/ Accepted 22 October 2003

The RdgC protein of Neisseria gonorrhoeae is required for efficient pilin antigenic variation, although its precise role has yet to be established. We demonstrate that the nearly identical RdgC from Neisseria meningitidis binds DNA with little specificity for sequence or structure, like the Escherichia coli protein. We also show that neither protein is able to constrain torsional tension in relaxed DNA. These data exclude several possible roles for RdgC in pilin antigenic variation and suggest that RdgC performs a similar function in both E. coli and the Neisseria spp.

* Corresponding author. Mailing address: Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom. Phone: 44 (0)115 9709406. Fax: 44 (0)115 9709906. E-mail: bob.lloyd{at}nottingham.ac.uk.

{dagger} Present address: Centre for Infectious Diseases, Wolfson Research Institute, University of Durham, Stockton-on-Tees TS15 6BH, United Kingdom.

Journal of Bacteriology, February 2004, p. 870-874, Vol. 186, No. 3
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.3.870-874.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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