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Journal of Bacteriology, March 2004, p. 1388-1397, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1388-1397.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Transcriptional Organization and Regulation of the L-Idonic Acid Pathway (GntII System) in Escherichia coli

Christoph Bausch,{dagger} Matthew Ramsey, and Tyrrell Conway*

Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019

Received 23 July 2003/ Accepted 19 November 2003

The genetic organization of the idn genes that encode the pathway for L-idonate catabolism was characterized. The monocistronic idnK gene is transcribed divergently from the idnDOTR genes, which were shown to form an operon. The 215-bp regulatory region between the idnK and idnD genes contains promoters in opposite orientation with transcription start sites that mapped to positions -26 and -29 with respect to the start codons. The regulatory region also contains a single putative IdnR/GntR binding site centered between the two promoters, a CRP binding site upstream of idnD, and an UP element upstream of idnK. The genes of the L-idonate pathway were shown to be under catabolite repression control. Analysis of idnD- and idnK-lacZ fusions in a nonpolar idnD mutant that is unable to interconvert L-idonate and 5-ketogluconate indicated that either compound could induce the pathway. The L-idonate pathway was first characterized as a subsidiary pathway for D-gluconate catabolism (GntII), which is induced by D-gluconate in a GntI (primary gluconate system) mutant. Here we showed that the idnK and idnD operons are induced by D-gluconate in a GntI system mutant, presumably by endogenous formation of 5-ketogluconate from D-gluconate. Thus, the regulation of the GntII system is appropriate for this pathway, which is primarily involved in L-idonate catabolism; the GntII system can be induced by D-gluconate under conditions that block the GntI system.

* Corresponding author. Mailing address: Advanced Center for Genome Technology, OU Microarray Core Facility, Department of Botany and Microbiology, 770 Van Vleet Oval, University of Oklahoma, Norman, OK 73019-0245. Phone: (405) 325-1683. Fax: (405) 325-7619. E-mail: tconway{at}ou.edu.

{dagger} Present address: Stowers Institute for Medical Research, Kansas City, MO 64110.

Journal of Bacteriology, March 2004, p. 1388-1397, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1388-1397.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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