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Journal of Bacteriology, March 2004, p. 1571-1573, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1571-1573.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Two Kinases Involved in Thiamine Pyrophosphate and Pyridoxal Phosphate Biosynthesis in Bacillus subtilis: 4-Amino-5-Hydroxymethyl-2-Methylpyrimidine Kinase and Pyridoxal Kinase

Joo-Heon Park, Kristin Burns, Cynthia Kinsland, and Tadhg P. Begley*

Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853

Received 29 May 2003/ Accepted 10 October 2003

Two Bacillus subtilis genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and B. subtilis ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.

* Corresponding author. Mailing address: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853. Phone: (607) 255-7133. Fax: (607) 255-4137. E-mail: tpb2{at}cornell.edu.

Journal of Bacteriology, March 2004, p. 1571-1573, Vol. 186, No. 5
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.5.1571-1573.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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