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Journal of Bacteriology, March 2004, p. 1678-1682, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1678-1682.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

DNA Amplification and Rearrangements in Archival Salmonella enterica Serovar Typhimurium LT2 Cultures

Steffen Porwollik,1 Rita Mei-Yee Wong,1 R. Allen Helm,2 Kelly K. Edwards,2 Michael Calcutt,2,3 Abraham Eisenstark,2,3* and Michael McClelland1

Sidney Kimmel Cancer Center, San Diego, California 92121,1 Cancer Research Center, Columbia, Missouri 65201,2 University of Missouri, Columbia, Missouri 652113

Received 21 August 2003/ Accepted 3 December 2003

Variations in genome size and gene order were observed in archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR analysis of the same strain revealed a genomic duplication that underwent a translocation. Other strains had smaller duplications and deletions. These results demonstrate that storage in stabs over time at room temperature not only allows for further bacterial growth but also may produce an environment that selects for a variety of mutations, including genomic rearrangements.

* Corresponding author. Mailing address: Cancer Research Center, 3501 Berrywood Dr., Columbia, MO 65201. Phone: (573) 875-2255. Fax: (573) 443-1202. E-mail: eisenstarka{at}missouri.edu.

Journal of Bacteriology, March 2004, p. 1678-1682, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1678-1682.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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