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Journal of Bacteriology, March 2004, p. 1729-1736, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1729-1736.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Light Control of hliA Transcription and Transcript Stability in the Cyanobacterium Synechococcus elongatus Strain PCC 7942

Kavitha Salem and Lorraine G. van Waasbergen*

Department of Biology and Converging Biotechnology Center, The University of Texas at Arlington, Arlington, Texas 76019

Received 14 August 2003/ Accepted 11 December 2003

The high-light-inducible proteins (HLIPs) of cyanobacteria are polypeptides involved in protecting the cells from high-intensity light (HL). The hliA gene encoding the HLIP from Synechococcus elongatus strain PCC 7942 is expressed in response to HL or low-intensity blue or UV-A light. In this study, we explore via Northern analysis details of the transcriptional regulation and transcript stability of the hliA gene under various light conditions. Transcript levels of the hliA gene increased dramatically upon a shift to HL or UV-A light to similar levels, followed by a rapid decrease in UV-A light, but not in HL, consistent with blue/UV-A light involvement in early stages of HL-mediated expression. A 3-min pulse of low-intensity UV-A light was enough to trigger hliA mRNA accumulation, indicating that a blue/UV-A photoreceptor is involved in upregulation of the gene. Low-intensity red light was found to cause a slight, transient increase in transcript levels (raising the possibility of red-light photoreceptor involvement), while light of other qualities had no apparent effect. No evidence was found for wavelength-specific attenuation of hliA transcript levels induced by HL or UV-A light. Transcript decay was slowed somewhat in darkness, and when photosynthetic electron transport was inhibited by darkness or treatment with DCMU, there appeared a smaller mRNA species that may represent a decay intermediate that accumulates when mRNA decay is slowed. Evidence suggests that upregulation of hliA by light is primarily a transcriptional response but conditions that cause ribosomes to stall on the transcript (e.g., a shift to darkness) can help stabilize hliA mRNA and affect expression levels.

* Corresponding author. Mailing address: Department of Biology and Converging Biotechnology Center, Box 19498, The University of Texas at Arlington, Arlington, TX 76019. Phone: (817) 272-5239. Fax: (817) 272-2855. E-mail: Lorivw{at}uta.edu.

Journal of Bacteriology, March 2004, p. 1729-1736, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1729-1736.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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