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Journal of Bacteriology, March 2004, p. 1890-1892, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1890-1892.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The eutD Gene of Salmonella enterica Encodes a Protein with Phosphotransacetylase Enzyme Activity

Shaun R. Brinsmade and Jorge C. Escalante-Semerena*

Department of Bacteriology, University of Wisconsin—Madison, Madison, Wisconsin

Received 17 November 2003/ Accepted 9 December 2003

The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.

* Corresponding author. Mailing address: Department of Bacteriology, University of Wisconsin—Madison, 264 Enzyme Institute, 1710 University Ave., Madison, WI 53726-4087. Phone: (608) 262-7379. Fax: (608) 265-7909. E-mail: escalante{at}bact.wisc.edu.

Journal of Bacteriology, March 2004, p. 1890-1892, Vol. 186, No. 6
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.6.1890-1892.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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