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Journal of Bacteriology, April 2004, p. 1933-1944, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.1933-1944.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Complete Genomic Sequence of the Virulent Salmonella Bacteriophage SP6

Aleisha T. Dobbins,1 Matthew George Jr.,1 Daryl A. Basham,1,{dagger} Michael E. Ford,2 Jennifer M. Houtz,2 Marisa L. Pedulla,2 Jeffrey G. Lawrence,2 Graham F. Hatfull,2 and Roger W. Hendrix2*

Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, D.C. 20059,1 Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 152602

Received 12 September 2003/ Accepted 10 December 2003

We report the complete genome sequence of enterobacteriophage SP6, which infects Salmonella enterica serovar Typhimurium. The genome contains 43,769 bp, including a 174-bp direct terminal repeat. The gene content and organization clearly place SP6 in the coliphage T7 group of phages, but there is ~5 kb at the right end of the genome that is not present in other members of the group, and the homologues of T7 genes 1.3 through 3 appear to have undergone an unusual reorganization. Sequence analysis identified 10 putative promoters for the SP6-encoded RNA polymerase and seven putative rho-independent terminators. The terminator following the gene encoding the major capsid subunit has a termination efficiency of about 50% with the SP6-encoded RNA polymerase. Phylogenetic analysis of phages related to SP6 provided clear evidence for horizontal exchange of sequences in the ancestry of these phages and clearly demarcated exchange boundaries; one of the recombination joints lies within the coding region for a phage exonuclease. Bioinformatic analysis of the SP6 sequence strongly suggested that DNA replication occurs in large part through a bidirectional mechanism, possibly with circular intermediates.

* Corresponding author. Mailing address: Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260. Phone: (412) 624-4674. Fax: (412) 624-4870. E-mail: rhx{at}pitt.edu.

{dagger} Present address: Gray Cary Ware & Freidenrich LLP, Washington, DC 20036.

Journal of Bacteriology, April 2004, p. 1933-1944, Vol. 186, No. 7
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.7.1933-1944.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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