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Journal of Bacteriology, April 2004, p. 2481-2486, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2481-2486.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel rpoB Mutations Conferring Rifampin Resistance on Bacillus subtilis: Global Effects on Growth, Competence, Sporulation, and Germination

Heather Maughan,1 Belinda Galeano,2 and Wayne L. Nicholson2*

Departments of Ecology and Evolutionary Biology,1 Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 857212

Received 11 September 2003/ Accepted 30 December 2003

Previously, spontaneous rifampin resistance mutations were isolated in cluster I of the rpoB gene, resulting in amino acid replacements (Q469R, H482R, H482Y, or S487L) in the Bacillus subtilis RNA polymerase ß subunit (W. L. Nicholson and H. Maughan, J. Bacteriol. 184:4936-4940, 2002). In this study, each amino acid change in the ß subunit was observed to result in its own unique spectrum of effects on growth and various developmental events, including sporulation, germination, and competence for transformation. The results thus establish the important role played by the RNA polymerase ß subunit, not only in the catalytic aspect of transcription, but also in the regulation of major developmental events in B. subtilis.

* Corresponding author. Present address: Department of Microbiology and Cell Science, University of Florida, Space Life Sciences Laboratory, Building M6-1025/SLSL, Kennedy Space Center, FL 32899. Phone: (321) 861-3487. Fax: (321) 861-2925. E-mail: WLN{at}ufl.edu.

Journal of Bacteriology, April 2004, p. 2481-2486, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2481-2486.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Perkins, A. E., Nicholson, W. L. (2008). Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants. J. Bacteriol. 190: 807-814 [Abstract] [Full Text]  
  • Mwangi, M. M., Wu, S. W., Zhou, Y., Sieradzki, K., de Lencastre, H., Richardson, P., Bruce, D., Rubin, E., Myers, E., Siggia, E. D., Tomasz, A. (2007). Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing. Proc. Natl. Acad. Sci. USA 104: 9451-9456 [Abstract] [Full Text]  


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