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Journal of Bacteriology, April 2004, p. 2508-2510, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2508-2510.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Clostridium difficile IStron CdISt1: Discovery of a Variant Encoding Two Complete Transposase-Like Proteins

Oliver Hasselmayer,1* Veit Braun,1 Claudia Nitsche,1 Michael Moos,1 Maja Rupnik,2 and Christoph von Eichel-Streiber1

Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University of Mainz, D-55101 Mainz,1 Department of Bacteriology, Georg-August-University Goettingen, D-37075 Goettingen, Germany2

Received 30 June 2003/ Accepted 21 November 2003

Screening a Clostridium difficile strain collection for the chimeric element CdISt1, we identified two additional variants, designated CdISt1-0 and CdISt1-III. In in vitro assays, we could prove the self-splicing ribozyme activity of these variants. Structural comparison of all known CdISt1 variants led us to define four types of IStrons that we designated CdISt1-0 through CdISt1-III. Since CdISt1-0 encodes two complete transposase-like proteins (TlpA and TlpB), we suggest that it represents the original genetic element, hypothesized before to have originated by fusion of a group I intron and an insertion sequence element.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University of Mainz, Obere Zahlbacher Strasse 67, D-55101 Mainz, Germany. Phone: 49 6131 39-32204. Fax: 49 6131 39-33364. E-mail: hasselm{at}mail.uni-mainz.de.

Journal of Bacteriology, April 2004, p. 2508-2510, Vol. 186, No. 8
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.8.2508-2510.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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