This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Pedro, M. A.
Right arrow Articles by Schwarz, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Pedro, M. A.
Right arrow Articles by Schwarz, H.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2004, p. 2594-2602, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2594-2602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Restricted Mobility of Cell Surface Proteins in the Polar Regions of Escherichia coli

Miguel A. de Pedro,1* Christoph G. Grünfelder,2 and Heinz Schwarz2

Centro de Biología Molecular "Severo Ochoa" Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain,1 Max-Planck Institut für Entwicklungsbiologie, 72076 Tübingen, Germany2

Received 4 September 2003/ Accepted 21 January 2004

The polar regions of the Escherichia coli murein sacculus are metabolically inert and stable in time. Because the sacculus and the outer membrane are tightly associated, we investigated whether polar inert murein could restrict the mobility of other cell envelope elements. Cells were covalently labeled with a fluorescent reagent, chased in dye-free medium, and observed by microscopy. Fluorescent material was more efficiently retained at the cell poles than at any other location. The boundary between high and low fluorescence intensity areas was rather sharp. Labeled material consisted mostly of cell envelope proteins, among them the free and murein-bound forms of Braun's lipoprotein. Our results indicate that the mobility of at least some cell envelope proteins is restrained at regions in correspondence with underlying areas of inert murein.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa" CSIC-UAM, Facultad de Ciencias UAM, Campus de Cantoblanco, 28049 Madrid, Spain. Phone: (34) 914978083. Fax: (34) 914978087. E-mail: madepedro{at}cbm.uam.es.

Journal of Bacteriology, May 2004, p. 2594-2602, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2594-2602.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Scheu, P., Sdorra, S., Liao, Y.-F., Wegner, M., Basche, T., Unden, G., Erker, W. (2008). Polar accumulation of the metabolic sensory histidine kinases DcuS and CitA in Escherichia coli. Microbiology 154: 2463-2472 [Abstract] [Full Text]  
  • Varma, A., de Pedro, M. A., Young, K. D. (2007). FtsZ Directs a Second Mode of Peptidoglycan Synthesis in Escherichia coli. J. Bacteriol. 189: 5692-5704 [Abstract] [Full Text]  
  • Priyadarshini, R., de Pedro, M. A., Young, K. D. (2007). Role of Peptidoglycan Amidases in the Development and Morphology of the Division Septum in Escherichia coli. J. Bacteriol. 189: 5334-5347 [Abstract] [Full Text]  
  • Young, K. D. (2006). The Selective Value of Bacterial Shape. Microbiol. Mol. Biol. Rev. 70: 660-703 [Abstract] [Full Text]  
  • Shih, Y.-L., Rothfield, L. (2006). The Bacterial Cytoskeleton. Microbiol. Mol. Biol. Rev. 70: 729-754 [Abstract] [Full Text]  
  • Leimanis, S., Hoyez, N., Hubert, S., Laschet, M., Sauvage, E., Brasseur, R., Coyette, J. (2006). PBP5 Complementation of a PBP3 Deficiency in Enterococcus hirae.. J. Bacteriol. 188: 6298-6307 [Abstract] [Full Text]  
  • Jain, S., van Ulsen, P., Benz, I., Schmidt, M. A., Fernandez, R., Tommassen, J., Goldberg, M. B. (2006). Polar Localization of the Autotransporter Family of Large Bacterial Virulence Proteins. J. Bacteriol. 188: 4841-4850 [Abstract] [Full Text]  
  • Zaritsky, A., Woldringh, C. L., Einav, M., Alexeeva, S. (2006). Use of thymine limitation and thymine starvation to study bacterial physiology and cytology.. J. Bacteriol. 188: 1667-1679 [Full Text]  
  • Scheffers, D.-J., Pinho, M. G. (2005). Bacterial Cell Wall Synthesis: New Insights from Localization Studies. Microbiol. Mol. Biol. Rev. 69: 585-607 [Abstract] [Full Text]  
  • Ghosh, A. S., Young, K. D. (2005). Helical Disposition of Proteins and Lipopolysaccharide in the Outer Membrane of Escherichia coli. J. Bacteriol. 187: 1913-1922 [Abstract] [Full Text]  
  • Wagner, J. K., Galvani, C. D., Brun, Y. V. (2005). Caulobacter crescentus Requires RodA and MreB for Stalk Synthesis and Prevention of Ectopic Pole Formation. J. Bacteriol. 187: 544-553 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»