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Journal of Bacteriology, May 2004, p. 2699-2707, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2699-2707.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Modulation of DNA Repair and Recombination by the Bacteriophage {lambda} Orf Function in Escherichia coli K-12

Anthony R. Poteete*

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Received 2 November 2003/ Accepted 23 January 2004

The orf gene of bacteriophage {lambda}, fused to a promoter, was placed in the galK locus of Escherichia coli K-12. Orf was found to suppress the recombination deficiency and sensitivity to UV radiation of mutants, in a {Delta}(recC ptr recB recD)::Ptac gam bet exo pae cI {Delta}recG background, lacking recF, recO, recR, ruvAB, and ruvC functions. It also suppressed defects of these mutants in establishing replication of a pSC101-related plasmid. Compared to orf, the recA803 allele had only small effects on recF, recO, and recR mutant phenotypes and no effect on a ruvAB mutant. In a fully wild-type background with respect to known recombination and repair functions, orf partially suppressed the UV sensitivity of ruvAB and ruvC mutants.

* Mailing address: Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655. Phone: (508) 856-3708. Fax: (508) 856-5920. E-mail: anthony.poteete{at}umassmed.edu.

Journal of Bacteriology, May 2004, p. 2699-2707, Vol. 186, No. 9
0021-9193/04/$08.00+0     DOI: 10.1128/JB.186.9.2699-2707.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Hayes, S., Asai, K., Chu, A. M., Hayes, C. (2005). NinR- and Red-Mediated Phage-Prophage Marker Rescue Recombination in Escherichia coli: Recovery of a Nonhomologous imm{lambda} DNA Segment by Infecting {lambda}imm434 Phages. Genetics 170: 1485-1499 [Abstract] [Full Text]  


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