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Journal of Bacteriology, May 2005, p. 3471-3476, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3471-3476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Operation of the CO Dehydrogenase/Acetyl Coenzyme A Pathway in both Acetate Oxidation and Acetate Formation by the Syntrophically Acetate-Oxidizing Bacterium Thermacetogenium phaeum

Satoshi Hattori,1,{dagger} Alexander S. Galushko,2 Yoichi Kamagata,1 and Bernhard Schink2*

Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan,1 Fakultät für Biologie, Universität Konstanz, Postfach 5560, 78457 Konstanz, Germany2

Received 27 July 2004/ Accepted 13 February 2005

Thermacetogenium phaeum is a homoacetogenic bacterium that can grow on various substrates, such as pyruvate, methanol, or H2/CO2. It can also grow on acetate if cocultured with the hydrogen-consuming methanogenic partner Methanothermobacter thermautotrophicus. Enzyme activities of the CO dehydrogenase/acetyl coenzyme A (CoA) pathway (CO dehydrogenase, formate dehydrogenase, formyl tetrahydrofolate synthase, methylene tetrahydrofolate dehydrogenase) were detected in cell extracts of pure cultures and of syntrophic cocultures. Mixed cell suspensions of T. phaeum and M. thermautotrophicus oxidized acetate rapidly and produced acetate after addition of H2/CO2 after a short time lag. CO dehydrogenase activity staining after native polyacrylamide gel electrophoresis exhibited three oxygen-labile bands which were identical in pure culture and coculture. Protein profiles of T. phaeum cells after sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the strain exhibited basically the same protein patterns in both pure and syntrophic culture. These results indicate that T. phaeum operates the CO dehydrogenase/acetyl-CoA pathway reversibly both in acetate oxidation and in reductive acetogenesis by using the same biochemical apparatus, although it has to couple this pathway to ATP synthesis in different ways.

* Corresponding author. Mailing address: Fakultät für Biologie, Universität Konstanz, Postfach 5560, 78457 Konstanz, Germany. Phone: 49-7531-882140. Fax: 49-7531-884047. E-mail: Bernhard.Schink{at}uni-konstanz.de.

{dagger} Present address: Environmental Molecular Biology Laboratory, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-0198, Japan.

Journal of Bacteriology, May 2005, p. 3471-3476, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3471-3476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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