This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Seto, S.
Right arrow Articles by Miyata, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seto, S.
Right arrow Articles by Miyata, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2005, p. 3502-3510, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3502-3510.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of a 521-Kilodalton Protein (Gli521) Involved in Force Generation or Force Transmission for Mycoplasma mobile Gliding

Shintaro Seto ,1,{dagger},{ddagger} Atsuko Uenoyama,1,{dagger} and Makoto Miyata1,2*

Department of Biology, Graduate School of Science, Osaka City University,1 PRESTO, JST, Sumiyoshi-ku, Osaka 558-8585, Japan2

Received 10 December 2004/ Accepted 7 February 2005

Several mycoplasma species are known to glide on solid surfaces such as glass in the direction of the membrane protrusion, but the mechanism underlying this movement is unknown. To identify a novel protein involved in gliding, we raised monoclonal antibodies against a detergent-insoluble protein fraction of Mycoplasma mobile, the fastest glider, and screened the antibodies for inhibitory effects on gliding. Five monoclonal antibodies stopped the movement of gliding mycoplasmas, keeping them on the glass surface, and all of them recognized a large protein in immunoblotting. This protein, named Gli521, is composed of 4,738 amino acids, has a predicted molecular mass of 520,559 Da, and is coded downstream of a gene for another gliding protein, Gli349, which is known to be responsible for glass binding during gliding. Edman degradation analysis indicated that the N-terminal region is processed at the peptide bond between the amino acid residues at positions 43 and 44. Analysis of gliding mutants isolated previously revealed that the Gli521 protein is missing in a nonbinding mutant, m9, where the gli521 gene is truncated by a nonsense mutation at the codon for the amino acid at position 1170. Immunofluorescence and immunoelectron microscopy indicated that Gli521 localizes all around the base of the membrane protrusion, at the "neck," as previously observed for Gli349. Analysis of the inhibitory effects of the anti-Gli521 antibody on gliding motility revealed that this protein is responsible for force generation or force transmission, a role distinct from that of Gli349, and also suggested conformational changes of Gli349 and Gli521 during gliding.

* Corresponding author. Mailing address: Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan. Phone: 81 (6) 6605 3157. Fax: 81 (6) 6605 3158. E-mail: miyata{at}sci.osaka-cu.ac.jp.

{dagger} S.S. and A.U. contributed equally to this work.

{ddagger} Present address: Division of Microbiology, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama, 350-0283, Japan.

Journal of Bacteriology, May 2005, p. 3502-3510, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3502-3510.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakane, D., Miyata, M. (2009). Cytoskeletal Asymmetrical Dumbbell Structure of a Gliding Mycoplasma, Mycoplasma gallisepticum, Revealed by Negative-Staining Electron Microscopy. J. Bacteriol. 191: 3256-3264 [Abstract] [Full Text]  
  • Uenoyama, A., Seto, S., Nakane, D., Miyata, M. (2009). Regions on Gli349 and Gli521 Protein Molecules Directly Involved in Movements of Mycoplasma mobile Gliding Machinery, Suggested by Use of Inhibitory Antibodies and Mutants. J. Bacteriol. 191: 1982-1985 [Abstract] [Full Text]  
  • Hatchel, J. M., Balish, M. F. (2008). Attachment organelle ultrastructure correlates with phylogeny, not gliding motility properties, in Mycoplasma pneumoniae relatives. Microbiology 154: 286-295 [Abstract] [Full Text]  
  • Nakane, D., Miyata, M. (2007). Cytoskeletal "jellyfish" structure of Mycoplasma mobile. Proc. Natl. Acad. Sci. USA 104: 19518-19523 [Abstract] [Full Text]  
  • Burgos, R., Pich, O. Q., Querol, E., Pinol, J. (2007). Functional Analysis of the Mycoplasma genitalium MG312 Protein Reveals a Specific Requirement of the MG312 N-Terminal Domain for Gliding Motility. J. Bacteriol. 189: 7014-7023 [Abstract] [Full Text]  
  • Hiratsuka, Y., Miyata, M., Tada, T., Uyeda, T. Q. P. (2006). A microrotary motor powered by bacteria. Proc. Natl. Acad. Sci. USA 103: 13618-13623 [Abstract] [Full Text]  
  • Hasselbring, B. M., Page, C. A., Sheppard, E. S., Krause, D. C. (2006). Transposon Mutagenesis Identifies Genes Associated with Mycoplasma pneumoniae Gliding Motility.. J. Bacteriol. 188: 6335-6345 [Abstract] [Full Text]  
  • Nagai, R., Miyata, M. (2006). Gliding Motility of Mycoplasma mobile Can Occur by Repeated Binding to N-Acetylneuraminyllactose (Sialyllactose) Fixed on Solid Surfaces.. J. Bacteriol. 188: 6469-6475 [Abstract] [Full Text]  
  • Hatchel, J. M., Balish, R. S., Duley, M. L., Balish, M. F. (2006). Ultrastructure and gliding motility of Mycoplasma amphoriforme, a possible human respiratory pathogen. Microbiology 152: 2181-2189 [Abstract] [Full Text]  
  • Adan-Kubo, J., Uenoyama, A., Arata, T., Miyata, M. (2006). Morphology of Isolated Gli349, a Leg Protein Responsible for Mycoplasma mobile Gliding via Glass Binding, Revealed by Rotary Shadowing Electron Microscopy.. J. Bacteriol. 188: 2821-2828 [Abstract] [Full Text]  
  • Braun, T. F., Khubbar, M. K., Saffarini, D. A., McBride, M. J. (2005). Flavobacterium johnsoniae Gliding Motility Genes Identified by mariner Mutagenesis. J. Bacteriol. 187: 6943-6952 [Abstract] [Full Text]  
  • Charon, N. W. (2005). Mycoplasma takes a walk. Proc. Natl. Acad. Sci. USA 102: 13713-13714 [Full Text]  
  • Hasselbring, B. M., Jordan, J. L., Krause, D. C. (2005). Mutant Analysis Reveals a Specific Requirement for Protein P30 in Mycoplasma pneumoniae Gliding Motility. J. Bacteriol. 187: 6281-6289 [Abstract] [Full Text]  
  • Uenoyama, A., Miyata, M. (2005). From the Cover: Gliding ghosts of Mycoplasma mobile. Proc. Natl. Acad. Sci. USA 102: 12754-12758 [Abstract] [Full Text]  
  • Uenoyama, A., Miyata, M. (2005). Identification of a 123-Kilodalton Protein (Gli123) Involved in Machinery for Gliding Motility of Mycoplasma mobile. J. Bacteriol. 187: 5578-5584 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»