This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Layton, A. N.
Right arrow Articles by Galyov, E. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Layton, A. N.
Right arrow Articles by Galyov, E. E.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2005, p. 3565-3571, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3565-3571.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Salmonella Translocated Effector SopA Is Targeted to the Mitochondria of Infected Cells

Abigail N. Layton, Philip J. Brown,{dagger} and Edouard E. Galyov*

Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom

Received 6 December 2004/ Accepted 3 February 2005

This study investigates the Salmonella effector protein SopA. We show that in Salmonella enterica serovar Dublin-infected cells, SopA1-347 fused to two carboxy-terminal hemagglutinin tags partially colocalized with mitochondria. Transfection of eukaryotic cells with a panel of constructs encoding truncated versions of SopA identified that amino acids 100 to 347 were sufficient to target SopA to the mitochondria.

* Corresponding author. Mailing address: Division of Microbiology, Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom. Phone: 44 (0)1635 578411. Fax: 44 (0)1635 577243. E-mail: edouard.galyov{at}bbsrc.ac.uk.

{dagger} Present address: Nuffield Department of Clinical Lab Sciences, University of Oxford, Level 4 Academic Block, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, United Kingdom.

Journal of Bacteriology, May 2005, p. 3565-3571, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3565-3571.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Giacomodonato, M. N., Uzzau, S., Bacciu, D., Caccuri, R., Sarnacki, S. H., Rubino, S., Cerquetti, M. C. (2007). SipA, SopA, SopB, SopD and SopE2 effector proteins of Salmonella enterica serovar Typhimurium are synthesized at late stages of infection in mice. Microbiology 153: 1221-1228 [Abstract] [Full Text]  
  • Rabin, S. D. P., Veesenmeyer, J. L., Bieging, K. T., Hauser, A. R. (2006). A C-Terminal Domain Targets the Pseudomonas aeruginosa Cytotoxin ExoU to the Plasma Membrane of Host Cells.. Infect. Immun. 74: 2552-2561 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»