This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kimura, Y.
Right arrow Articles by Takegawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kimura, Y.
Right arrow Articles by Takegawa, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, May 2005, p. 3593-3598, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3593-3598.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

An Adenylyl Cyclase, CyaB, Acts as an Osmosensor in Myxococcus xanthus

Yoshio Kimura,* Mika Ohtani, and Kaoru Takegawa

Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, Japan 761-0795

Received 1 November 2004/ Accepted 4 February 2005

We have previously reported that a receptor-type adenylyl cyclase (CyaA) of Myxococcus xanthus undergoes an osmosensor mainly during spore germination (Y. Kimura et al., J. Bacteriol. 184:3578-3585, 2002). In the present study, we cloned another receptor-type adenylyl cyclase gene (cyaB) and characterized the function of the cyaB-encoded protein. Disruption of cyaB generates a mutant that showed growth retardation at high ionic (NaCl) or high nonionic (sucrose) osmolarity. When vegetative cells were stimulated with 0.15 M NaCl, the increases in intracellular cyclic AMP levels of cyaB mutant cells were lower than those of wild-type cells. Under nonionic osmostress, the cyaB mutant exhibited reduced spore germination; however, the germination rate of the cyaB mutant was significantly higher than that of the cyaA mutant.

* Corresponding author. Mailing address: Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, Japan 761-0795. Phone: 81-87-891-3118. Fax: 81-87-891-3021. E-mail: kimura{at}ag.kagawa-u.ac.jp.

Journal of Bacteriology, May 2005, p. 3593-3598, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3593-3598.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Matsunaga, J., Medeiros, M. A., Sanchez, Y., Werneid, K. F., Ko, A. I. (2007). Osmotic regulation of expression of two extracellular matrix-binding proteins and a haemolysin of Leptospira interrogans: differential effects on LigA and Sph2 extracellular release. Microbiology 153: 3390-3398 [Abstract] [Full Text]  
  • Bonner, P. J., Shimkets, L. J. (2006). Cohesion-Defective Mutants of Myxococcus xanthus.. J. Bacteriol. 188: 4585-4588 [Abstract] [Full Text]  
  • Kimura, Y., Nakatuma, H., Sato, N., Ohtani, M. (2006). Contribution of the Cyclic Nucleotide Phosphodiesterases PdeA and PdeB to Adaptation of Myxococcus xanthus Cells to Osmotic or High-Temperature Stress. J. Bacteriol. 188: 823-828 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»