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Journal of Bacteriology, May 2005, p. 3599-3601, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3599-3601.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Localization of Chaperones DnaK and GroEL in Bacterial Inclusion Bodies

M. Mar Carrió and Antonio Villaverde*

Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain

Received 10 January 2005/ Accepted 31 January 2005

By immunostaining and transmission electron microscopy, chaperones DnaK and GroEL have been identified at the solvent-exposed surface of bacterial inclusion bodies and entrapped within these aggregates, respectively. Functional implications of this distinct localization are discussed in the context of Escherichia coli protein quality control.


* Corresponding author. Mailing address: Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. Phone: 34 935812148. Fax: 34 935812011. E-mail: avillaverde{at}servet.uab.es.


Journal of Bacteriology, May 2005, p. 3599-3601, Vol. 187, No. 10
0021-9193/05/$08.00+0     doi:10.1128/JB.187.10.3599-3601.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.




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