This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dedysh, S. N.
Right arrow Articles by Trotsenko, Y. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dedysh, S. N.
Right arrow Articles by Trotsenko, Y. A.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2005, p. 3884-3888, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3884-3888.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Methylotrophic Autotrophy in Beijerinckia mobilis

Svetlana N. Dedysh,1* Ksenia V. Smirnova,2 Valentina N. Khmelenina,2 Natalia E. Suzina,2 Werner Liesack,3 and Yuri A. Trotsenko2

S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia,1 G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow 142290, Russia,2 Max-Planck-Institut für Terrestrische Mikrobiologie, D-35043 Marburg, Germany3

Received 10 November 2004/ Accepted 21 February 2005

Representatives of the genus Beijerinckia are known as heterotrophic, dinitrogen-fixing bacteria which utilize a wide range of multicarbon compounds. Here we show that at least one of the currently known species of this genus, i.e., Beijerinckia mobilis, is also capable of methylotrophic metabolism coupled with the ribulose bisphosphate (RuBP) pathway of C1 assimilation. A complete suite of dehydrogenases commonly involved in the sequential oxidation of methanol via formaldehyde and formate to CO2 was detected in cell extracts of B. mobilis grown on CH3OH. Carbon dioxide produced by oxidation of methanol was further assimilated via the RuBP pathway as evidenced by reasonably high activities of phosphoribulokinase and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). Detection and partial sequence analysis of genes encoding the large subunits of methanol dehydrogenase (mxaF) and form I RubisCO (cbbL) provided genotypic evidence for methylotrophic autotrophy in B. mobilis.

* Corresponding author. Mailing address: S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia. Phone: 7 (095) 135 0591. Fax: 7 (095) 135 6530. E-mail: s.dedysh{at}mtu-net.ru.

Journal of Bacteriology, June 2005, p. 3884-3888, Vol. 187, No. 11
0021-9193/05/$08.00+0     doi:10.1128/JB.187.11.3884-3888.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vorob'ev, A. V., de Boer, W., Folman, L. B., Bodelier, P. L. E., Doronina, N. V., Suzina, N. E., Trotsenko, Y. A., Dedysh, S. N. (2009). Methylovirgula ligni gen. nov., sp. nov., an obligately acidophilic, facultatively methylotrophic bacterium with a highly divergent mxaF gene. Int. J. Syst. Evol. Microbiol. 59: 2538-2545 [Abstract] [Full Text]  
  • Rothschild, L. J (2008). The evolution of photosynthesis...again?. Phil Trans R Soc B 363: 2787-2801 [Abstract] [Full Text]  
  • Dick, G. J., Podell, S., Johnson, H. A., Rivera-Espinoza, Y., Bernier-Latmani, R., McCarthy, J. K., Torpey, J. W., Clement, B. G., Gaasterland, T., Tebo, B. M. (2008). Genomic Insights into Mn(II) Oxidation by the Marine Alphaproteobacterium Aurantimonas sp. Strain SI85-9A1. Appl. Environ. Microbiol. 74: 2646-2658 [Abstract] [Full Text]  
  • Sorokin, D. Yu., Trotsenko, Y. A., Doronina, N. V., Tourova, T. P., Galinski, E. A., Kolganova, T. V., Muyzer, G. (2007). Methylohalomonas lacus gen. nov., sp. nov. and Methylonatrum kenyense gen. nov., sp. nov., methylotrophic gammaproteobacteria from hypersaline lakes. Int. J. Syst. Evol. Microbiol. 57: 2762-2769 [Abstract] [Full Text]  
  • Kulichevskaya, I. S., Guzev, V. S., Gorlenko, V. M., Liesack, W., Dedysh, S. N. (2006). Rhodoblastus sphagnicola sp. nov., a novel acidophilic purple non-sulfur bacterium from Sphagnum peat bog. Int. J. Syst. Evol. Microbiol. 56: 1397-1402 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»