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Journal of Bacteriology, June 2005, p. 3997-4004, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.3997-4004.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Peculiar Properties of DsbA in Its Export across the Escherichia coli Cytoplasmic Membrane

Nobuyuki Shimohata, Yoshinori Akiyama, and Koreaki Ito*

Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

Received 18 November 2004/ Accepted 8 March 2005

Export of DsbA, a protein disulfide bond-introducing enzyme, across the Escherichia coli cytoplasmic membrane was studied with special reference to the effects of various mutations affecting translocation factors. It was noted that both the internalized precursor retaining the signal peptide and the periplasmic mature product fold rapidly into a protease-resistant structure and they exhibited anomalies in sodium dodecyl sulfate-polyacrylamide gel electrophoresis in that the former migrated faster than the latter. The precursor, once accumulated, was not exported posttranslationally. DsbA export depended on the SecY translocon, the SecA ATPase, and Ffh (signal recognition particle), but not on SecB. SecY mutations, such as secY39 and secY205, that severely impair translocation of a number of secretory substrates by interfering with SecA actions only insignificantly impaired the DsbA export. In contrast, secY125, affecting a periplasmic domain and impairing a late step of translocation, exerted strong export inhibition of both classes of proteins. These results suggest that DsbA uses not only the signal recognition particle targeting pathway but also a special route of translocation through the translocon, which is hence suggested to actively discriminate preproteins.

* Correspondence author. Mailing address: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan. Phone: 81-75-751-4015. Fax: 81-75-771-5699. E-mail: kito{at}virus.kyoto-u.ac.jp.

Journal of Bacteriology, June 2005, p. 3997-4004, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.3997-4004.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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