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Journal of Bacteriology, June 2005, p. 4005-4014, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.4005-4014.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of the Small Untranslated RNA RyhB and Its Regulon in Vibrio cholerae{dagger}

Brigid M. Davis,2* Mariam Quinones,2 Jason Pratt,2 Yanpeng Ding,2,{ddagger} and Matthew K. Waldor1,2

Howard Hughes Medical Institute,1 Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, Massachusetts 021112

Received 23 November 2004/ Accepted 3 March 2005

Numerous small untranslated RNAs (sRNAs) have been identified in Escherichia coli in recent years, and their roles are gradually being defined. However, few of these sRNAs appear to be conserved in Vibrio cholerae, and both identification and characterization of sRNAs in V. cholerae remain at a preliminary stage. We have characterized one of the few sRNAs conserved between E. coli and V. cholerae: RyhB. Sequence conservation is limited to the central region of the gene, and RyhB in V. cholerae is significantly larger than in E. coli. As in E. coli, V. cholerae RyhB is regulated by the iron-dependent repressor Fur, and it interacts with the RNA-binding protein Hfq. The regulons controlled by RyhB in V. cholerae and E. coli appear to differ, although some overlap is evident. Analysis of gene expression in V. cholerae in the absence of RyhB suggests that the role of this sRNA is not limited to control of iron utilization. Quantitation of RyhB expression in the suckling mouse intestine suggests that iron availability is not limiting in this environment, and RyhB is not required for colonization of this mammalian host by V. cholerae.

* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-2778. Fax: (617) 636-2723. E-mail: brigid.davis{at}tufts.edu.

{dagger} Supplementary material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Tufts-NEMC, 750 Washington St., Box 041, Boston, MA 02111.

Journal of Bacteriology, June 2005, p. 4005-4014, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.4005-4014.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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