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Journal of Bacteriology, June 2005, p. 4198-4206, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.4198-4206.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Second Outer-Core Region in Klebsiella pneumoniae Lipopolysaccharide{dagger}

Miguel Regué,1 Luis Izquierdo,2 Sandra Fresno,2 Núria Piqué,1 Maria Michela Corsaro,3 Teresa Naldi,3 Cristina De Castro,3 Dietmar Waidelich,4 Susana Merino,2 and Juan M. Tomás2*

Departamento de Microbiología i Parasitología Sanitarias, Facultad de Farmacia, Universidad de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain,1 Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08028 Barcelona, Spain,2 Dipartimento di Chimica Organica e Biochimica, Università Federico II di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy,3 Applied Biosystems, Frankfurter Strasse 129B, 64293 Darmstadt, Germany4

Received 28 February 2005/ Accepted 11 March 2005

Up to now only one major type of core oligosaccharide has been found in the lipopolysaccharide of all Klebsiella pneumoniae strains analyzed. Applying a different screening approach, we identified a novel Klebsiella pneumoniae core (type 2). Both Klebsiella core types share the same inner core and the outer-core-proximal disaccharide, GlcN-(1,4)-GalA, but they differ in the GlcN substituents. In core type 2, the GlcpN residue is substituted at the O-4 position by the disaccharide ß-Glcp(1-6)-{alpha}-Glcp(1, while in core type 1 the GlcpN residue is substituted at the O-6 position by either the disaccharide {alpha}-Hep(1-4)-{alpha}-Kdo(2 or a Kdo residue (Kdo is 3-deoxy-D-manno-octulosonic acid). This difference correlates with the presence of a three-gene region in the corresponding core biosynthetic clusters. Engineering of both core types by interchanging this specific region allowed studying the effect on virulence. The replacement of Klebsiella core type 1 in a highly type 2 virulent strain (52145) induces lower virulence than core type 2 in a murine infection model.

* Corresponding author. Mailing address: Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08028 Barcelona, Spain. Phone: 34-934021486. Fax: 34-93-4039047. E-mail: jtomas{at}ub.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, June 2005, p. 4198-4206, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.4198-4206.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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