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Journal of Bacteriology, June 2005, p. 4290-4294, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.4290-4294.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Transcription Termination Factor Rho Is Essential and Autoregulated in Caulobacter crescentus

Valéria C. S. Italiani and Marilis V. Marques*

Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, SP 05508-900, Brazil

Received 22 December 2004/ Accepted 11 March 2005

The impossibility of obtaining a rho null mutant and sensitivity to bicyclomycin have indicated that rho is essential for the viability of Caulobacter crescentus. Transcription gene fusions of sequences with serial deletions of the rho 5' untranslated region (5'-UTR) with a lacZ reporter gene indicated that rho is autoregulated at the level of attenuation of transcription in the 5'-UTR.

* Corresponding author. Mailing address: Av. Prof. Lineu Prestes, 1374, 05508-900 São Paulo, SP, Brazil. Phone: 55-11-3091-7299. Fax: 55-11-3091-7354. E-mail: mvmarque{at}usp.br.

Journal of Bacteriology, June 2005, p. 4290-4294, Vol. 187, No. 12
0021-9193/05/$08.00+0     doi:10.1128/JB.187.12.4290-4294.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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