This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Navarro-Llorens, J. M.
Right arrow Articles by Mohn, W. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Navarro-Llorens, J. M.
Right arrow Articles by Mohn, W. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2005, p. 4497-4504, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4497-4504.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Phenylacetate Catabolism in Rhodococcus sp. Strain RHA1: a Central Pathway for Degradation of Aromatic Compounds

Juana María Navarro-Llorens,1 Marianna A. Patrauchan,2 Gordon R. Stewart,2 Julian E. Davies,2 Lindsay D. Eltis,2 and William W. Mohn2*

Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, 28040 Madrid, Spain,1 Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Blvd., Vancouver, BC V6T 1Z3, Canada2

Received 15 November 2004/ Accepted 16 March 2005

In gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (PAA) that proceeds via phenylacetyl-coenzyme A (CoA) and hydrolytic ring fission plays a central role in the degradation of a range of aromatic compounds. In contrast, the PAA pathway and its role are not well characterized in gram-positive bacteria. A cluster including 13 paa genes encoding enzymes orthologous to those of gram-negative bacteria was identified on the chromosome of Rhodococcus sp. strain RHA1. These genes were transcribed during growth on PAA, with 11 of the genes apparently in an operon yielding a single transcript. Quantitative proteomic analyses revealed that at least 146 proteins were more than twice as abundant in PAA-grown cells of RHA1 than in pyruvate-grown cells. Of these proteins, 29 were identified, including 8 encoded by the paa genes. Knockout mutagenesis indicated that paaN, encoding a putative ring-opening enzyme, was essential for growth on PAA. However, paaF, encoding phenylacetyl-CoA ligase, and paaR, encoding a putative regulator, were not essential. paaN was also essential for growth of RHA1 on phenylacetaldehyde, phenylpyruvate, 4-phenylbutyrate, 2-phenylethanol, 2-phenylethylamine, and L-phenylalanine. In contrast, growth on 3-hydroxyphenylacetate, ethylbenzene, and styrene was unaffected. These results suggest that the range of substrates degraded via the PAA pathway in RHA1 is somewhat limited relative to the range in previously characterized gram-negative bacteria.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Blvd., Vancouver, BC V6T 1Z3, Canada. Phone: (604) 822-4285. Fax: (604) 822-6041. E-mail: wmohn{at}interchange.ubc.ca.

Journal of Bacteriology, July 2005, p. 4497-4504, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4497-4504.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Law, R. J., Hamlin, J. N. R., Sivro, A., McCorrister, S. J., Cardama, G. A., Cardona, S. T. (2008). A Functional Phenylacetic Acid Catabolic Pathway Is Required for Full Pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans Host Model. J. Bacteriol. 190: 7209-7218 [Abstract] [Full Text]  
  • Patrauchan, M. A., Florizone, C., Eapen, S., Gomez-Gil, L., Sethuraman, B., Fukuda, M., Davies, J., Mohn, W. W., Eltis, L. D. (2008). Roles of Ring-Hydroxylating Dioxygenases in Styrene and Benzene Catabolism in Rhodococcus jostii RHA1. J. Bacteriol. 190: 37-47 [Abstract] [Full Text]  
  • Nogales, J., Macchi, R., Franchi, F., Barzaghi, D., Fernandez, C., Garcia, J. L., Bertoni, G., Diaz, E. (2007). Characterization of the last step of the aerobic phenylacetic acid degradation pathway. Microbiology 153: 357-365 [Abstract] [Full Text]  
  • Fernandez, C., Ferrandez, A., Minambres, B., Diaz, E., Garcia, J. L. (2006). Genetic Characterization of the Phenylacetyl-Coenzyme A Oxygenase from the Aerobic Phenylacetic Acid Degradation Pathway of Escherichia coli. Appl. Environ. Microbiol. 72: 7422-7426 [Abstract] [Full Text]  
  • McLeod, M. P., Warren, R. L., Hsiao, W. W. L., Araki, N., Myhre, M., Fernandes, C., Miyazawa, D., Wong, W., Lillquist, A. L., Wang, D., Dosanjh, M., Hara, H., Petrescu, A., Morin, R. D., Yang, G., Stott, J. M., Schein, J. E., Shin, H., Smailus, D., Siddiqui, A. S., Marra, M. A., Jones, S. J. M., Holt, R., Brinkman, F. S. L., Miyauchi, K., Fukuda, M., Davies, J. E., Mohn, W. W., Eltis, L. D. (2006). The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc. Natl. Acad. Sci. USA 103: 15582-15587 [Abstract] [Full Text]  
  • Goncalves, E. R., Hara, H., Miyazawa, D., Davies, J. E., Eltis, L. D., Mohn, W. W. (2006). Transcriptomic Assessment of Isozymes in the Biphenyl Pathway of Rhodococcus sp. Strain RHA1. Appl. Environ. Microbiol. 72: 6183-6193 [Abstract] [Full Text]  
  • del Peso-Santos, T., Bartolome-Martin, D., Fernandez, C., Alonso, S., Garcia, J. L., Diaz, E., Shingler, V., Perera, J. (2006). Coregulation by Phenylacetyl-Coenzyme A-Responsive PaaX Integrates Control of the Upper and Lower Pathways for Catabolism of Styrene by Pseudomonas sp. Strain Y2. J. Bacteriol. 188: 4812-4821 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»