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Journal of Bacteriology, July 2005, p. 4598-4606, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4598-4606.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genetic Evidence that GTP Is Required for Transposition of IS903 and Tn552 in Escherichia coli

Abbie M. Coros,1 Erin Twiss,1,2 Norma P. Tavakoli,1 and Keith M. Derbyshire1,2*

Division of Infectious Disease, Wadsworth Center, New York State Department of Health,1 Department of Biomedical Sciences, University at Albany, Albany, New York 122012

Received 19 January 2005/ Accepted 28 March 2005

Surprisingly little is known about the role of host factors in regulating transposition, despite the potentially deleterious rearrangements caused by the movement of transposons. An extensive mutant screen was therefore conducted to identify Escherichia coli host factors that regulate transposition. An E. coli mutant library was screened using a papillation assay that allows detection of IS903 transposition events by the formation of blue papillae on a colony. Several host mutants were identified that exhibited a unique papillation pattern: a predominant ring of papillae just inside the edge of the colony, implying that transposition was triggered within these cells based on their spatial location within the colony. These mutants were found to be in pur genes, whose products are involved in the purine biosynthetic pathway. The transposition ring phenotype was also observed with Tn552, but not Tn10, establishing that this was not unique to IS903 and that it was not an artifact of the assay. Further genetic analyses of purine biosynthetic mutants indicated that the ring of transposition was consistent with a GTP requirement for IS903 and Tn552 transposition. Together, our observations suggest that transposition occurs during late stages of colony growth and that transposition occurs inside the colony edge in response to both a gradient of exogenous purines across the colony and the developmental stage of the cells.

* Corresponding author. Mailing address: David Axelrod Institute, CMS4217, NYS Department of Health, P.O. Box 22002, Albany, NY 12201-2002. Phone: (518) 473-6079. Fax: (518) 402-2633. E-mail: keith.derbyshire{at}wadsworth.org.

Journal of Bacteriology, July 2005, p. 4598-4606, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4598-4606.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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