This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gaspar, A. H.
Right arrow Articles by Schneewind, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaspar, A. H.
Right arrow Articles by Schneewind, O.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2005, p. 4646-4655, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4646-4655.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bacillus anthracis Sortase A (SrtA) Anchors LPXTG Motif-Containing Surface Proteins to the Cell Wall Envelope

Andrew H. Gaspar,1,{dagger} Luciano A. Marraffini,2 Elizabeth M. Glass,3 Kristin L. DeBord,1 Hung Ton-That,1,{dagger} and Olaf Schneewind1*

Department of Microbiology,1 Department of Molecular Genetics & Cell Biology, University of Chicago, 920 East 58th Street, Chicago, Illinois,2 Mathematics and Computer Sciences Division, Argonne National Laboratory, Argonne, Illinois3

Received 22 January 2005/ Accepted 25 March 2005

Cell wall-anchored surface proteins of gram-positive pathogens play important roles during the establishment of many infectious diseases, but the contributions of surface proteins to the pathogenesis of anthrax have not yet been revealed. Cell wall anchoring in Staphylococcus aureus occurs by a transpeptidation mechanism requiring surface proteins with C-terminal sorting signals as well as sortase enzymes. The genome sequence of Bacillus anthracis encodes three sortase genes and eleven surface proteins with different types of cell wall sorting signals. Purified B. anthracis sortase A cleaved peptides encompassing LPXTG motif-type sorting signals between the threonine (T) and the glycine (G) residues in vitro. Sortase A activity could be inhibited by thiol-reactive reagents, similar to staphylococcal sortases. B. anthracis parent strain Sterne 34F2, but not variants lacking the srtA gene, anchored the collagen-binding MSCRAMM (microbial surface components recognizing adhesive matrix molecules) BasC (BA5258/BAS4884) to the bacterial cell wall. These results suggest that B. anthracis SrtA anchors surface proteins bearing LPXTG motif sorting signals to the cell wall envelope of vegetative bacilli.

* Corresponding author. Mailing address: Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637. Phone: (773) 834 9060. Fax: (773) 834-8150. E-mail: oschnee{at}bsd.uchicago.edu.

{dagger} Present address: Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030.

Journal of Bacteriology, July 2005, p. 4646-4655, Vol. 187, No. 13
0021-9193/05/$08.00+0     doi:10.1128/JB.187.13.4646-4655.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Turner, L. S., Kanamoto, T., Unoki, T., Munro, C. L., Wu, H., Kitten, T. (2009). Comprehensive Evaluation of Streptococcus sanguinis Cell Wall-Anchored Proteins in Early Infective Endocarditis. Infect. Immun. 77: 4966-4975 [Abstract] [Full Text]  
  • Thammavongsa, V., Kern, J. W., Missiakas, D. M., Schneewind, O. (2009). Staphylococcus aureus synthesizes adenosine to escape host immune responses. JEM 206: 2417-2427 [Abstract] [Full Text]  
  • Budzik, J. M., Oh, S.-Y., Schneewind, O. (2009). Sortase D Forms the Covalent Bond That Links BcpB to the Tip of Bacillus cereus Pili. J. Biol. Chem. 284: 12989-12997 [Abstract] [Full Text]  
  • Mariscotti, J. F., Portillo, F. G.-d., Pucciarelli, M. G. (2009). The Listeria monocytogenes Sortase-B Recognizes Varied Amino Acids at Position 2 of the Sorting Motif. J. Biol. Chem. 284: 6140-6146 [Abstract] [Full Text]  
  • Budzik, J. M., Oh, S.-Y., Schneewind, O. (2008). Cell Wall Anchor Structure of BcpA Pili in Bacillus anthracis. J. Biol. Chem. 283: 36676-36686 [Abstract] [Full Text]  
  • Garufi, G., Butler, E., Missiakas, D. (2008). ESAT-6-Like Protein Secretion in Bacillus anthracis. J. Bacteriol. 190: 7004-7011 [Abstract] [Full Text]  
  • Marraffini, L. A., Schneewind, O. (2007). Sortase C-Mediated Anchoring of BasI to the Cell Wall Envelope of Bacillus anthracis. J. Bacteriol. 189: 6425-6436 [Abstract] [Full Text]  
  • Maresso, A. W., Chapa, T. J., Schneewind, O. (2006). Surface Protein IsdC and Sortase B Are Required for Heme-Iron Scavenging of Bacillus anthracis. J. Bacteriol. 188: 8145-8152 [Abstract] [Full Text]  
  • Gat, O., Grosfeld, H., Ariel, N., Inbar, I., Zaide, G., Broder, Y., Zvi, A., Chitlaru, T., Altboum, Z., Stein, D., Cohen, S., Shafferman, A. (2006). Search for Bacillus anthracis Potential Vaccine Candidates by a Functional Genomic-Serologic Screen. Infect. Immun. 74: 3987-4001 [Abstract] [Full Text]  
  • LeMieux, J., Hava, D. L., Basset, A., Camilli, A. (2006). RrgA and RrgB Are Components of a Multisubunit Pilus Encoded by the Streptococcus pneumoniae rlrA Pathogenicity Islet. Infect. Immun. 74: 2453-2456 [Abstract] [Full Text]  
  • Marraffini, L. A., DeDent, A. C., Schneewind, O. (2006). Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 70: 192-221 [Abstract] [Full Text]  
  • Davison, S., Couture-Tosi, E., Candela, T., Mock, M., Fouet, A. (2005). Identification of the Bacillus anthracis {gamma} Phage Receptor. J. Bacteriol. 187: 6742-6749 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»