This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vepachedu, V. R.
Right arrow Articles by Setlow, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vepachedu, V. R.
Right arrow Articles by Setlow, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 2005, p. 5677-5682, Vol. 187, No. 16
0021-9193/05/$08.00+0     doi:10.1128/JB.187.16.5677-5682.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Localization of SpoVAD to the Inner Membrane of Spores of Bacillus subtilis

Venkata Ramana Vepachedu and Peter Setlow*

Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06032

Received 5 April 2005/ Accepted 24 May 2005

The products of the hexacistronic spoVA operon of Bacillus subtilis may be involved in the transport of dipicolinic acid into the forespore during sporulation and its release during spore germination. The major hydrophilic coding region of B. subtilis spoVAD was cloned, the protein was expressed in Escherichia coli as a His tag fusion protein, and a rabbit antiserum was raised against the purified protein. Western blot analyses of fractions from B. subtilis spores showed that SpoVAD is an integral inner membrane protein present at levels >50-fold higher than those of the spore's nutrient germinant receptors that are also present in the inner membrane. SpoVAD also persisted in outgrowing spores.

* Corresponding author. Mailing address: Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032. Phone: (860) 679-2607. Fax: (860) 679-3408. E-mail: setlow{at}nso2.uchc.edu.

Journal of Bacteriology, August 2005, p. 5677-5682, Vol. 187, No. 16
0021-9193/05/$08.00+0     doi:10.1128/JB.187.16.5677-5682.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Paredes-Sabja, D., Setlow, P., Sarker, M. R. (2009). GerO, a Putative Na+/H+-K+ Antiporter, Is Essential for Normal Germination of Spores of the Pathogenic Bacterium Clostridium perfringens. J. Bacteriol. 191: 3822-3831 [Abstract] [Full Text]  
  • Ghosh, S., Setlow, P. (2009). Isolation and Characterization of Superdormant Spores of Bacillus Species. J. Bacteriol. 191: 1787-1797 [Abstract] [Full Text]  
  • Pelczar, P. L., Setlow, P. (2008). Localization of the Germination Protein GerD to the Inner Membrane in Bacillus subtilis Spores. J. Bacteriol. 190: 5635-5641 [Abstract] [Full Text]  
  • Paredes-Sabja, D., Bond, C., Carman, R. J., Setlow, P., Sarker, M. R. (2008). Germination of spores of Clostridium difficile strains, including isolates from a hospital outbreak of Clostridium difficile-associated disease (CDAD). Microbiology 154: 2241-2250 [Abstract] [Full Text]  
  • Paredes-Sabja, D., Setlow, B., Setlow, P., Sarker, M. R. (2008). Characterization of Clostridium perfringens Spores That Lack SpoVA Proteins and Dipicolinic Acid. J. Bacteriol. 190: 4648-4659 [Abstract] [Full Text]  
  • Paredes-Sabja, D., Torres, J. A., Setlow, P., Sarker, M. R. (2008). Clostridium perfringens Spore Germination: Characterization of Germinants and Their Receptors. J. Bacteriol. 190: 1190-1201 [Abstract] [Full Text]  
  • Cote, C. K., Bozue, J., Moody, K. L., DiMezzo, T. L., Chapman, C. E., Welkos, S. L. (2008). Analysis of a novel spore antigen in Bacillus anthracis that contributes to spore opsonization. Microbiology 154: 619-632 [Abstract] [Full Text]  
  • Vepachedu, V. R., Setlow, P. (2007). Role of SpoVA Proteins in Release of Dipicolinic Acid during Germination of Bacillus subtilis Spores Triggered by Dodecylamine or Lysozyme. J. Bacteriol. 189: 1565-1572 [Abstract] [Full Text]  
  • Atluri, S., Ragkousi, K., Cortezzo, D. E., Setlow, P. (2006). Cooperativity Between Different Nutrient Receptors in Germination of Spores of Bacillus subtilis and Reduction of This Cooperativity by Alterations in the GerB Receptor. J. Bacteriol. 188: 28-36 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»