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Journal of Bacteriology, September 2005, p. 6005-6018, Vol. 187, No. 17
0021-9193/05/$08.00+0     doi:10.1128/JB.187.17.6005-6018.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

E1 Enzyme of the Pyruvate Dehydrogenase Complex in Corynebacterium glutamicum: Molecular Analysis of the Gene and Phylogenetic Aspects

Mark E. Schreiner, Diana Fiur, Jirí Holátko,{dagger} Miroslav Pátek,{dagger} and Bernhard J. Eikmanns*

Department of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany

Received 22 March 2005/ Accepted 10 June 2005

The E1p enzyme is an essential part of the pyruvate dehydrogenase complex (PDHC) and catalyzes the oxidative decarboxylation of pyruvate with concomitant acetylation of the E2p enzyme within the complex. We analyzed the Corynebacterium glutamicum aceE gene, encoding the E1p enzyme, and constructed and characterized an E1p-deficient mutant. Sequence analysis of the C. glutamicum aceE gene and adjacent regions revealed that aceE is not flanked by genes encoding other enzymes of the PDHC. Transcriptional analysis revealed that aceE from C. glutamicum is monocistronic and that its transcription is initiated 121 nucleotides upstream of the translational start site. Inactivation of the chromosomal aceE gene led to the inability to grow on glucose and to the absence of PDHC and E1p activities, indicating that only a single E1p enzyme is present in C. glutamicum and that the PDHC is essential for the growth of this organism on carbohydrate substrates. Surprisingly, the E1p enzyme of C. glutamicum showed up to 51% identity to homodimeric E1p proteins from gram-negative bacteria but no similarity to E1 {alpha}- or ß-subunits of heterotetrameric E1p enzymes which are generally assumed to be typical for gram-positives. To investigate the distribution of E1p enzymes in bacteria, we compiled and analyzed the phylogeny of 46 homodimeric E1p proteins and of 58 {alpha}-subunits of heterotetrameric E1p proteins deposited in public databases. The results revealed that the distribution of homodimeric and heterotetrameric E1p subunits in bacteria is not in accordance with the rRNA-based phylogeny of bacteria and is more heterogeneous than previously assumed.

* Corresponding author. Mailing address: Department of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany. Phone: 49 (0) 731 50 22707. Fax: 49 (0) 731 50 22719. E-mail: bernhard.eikmanns{at}biologie.uni-ulm.de.

{dagger} Present address: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, CZ-14220 Prague 4, Czech Republic.

Journal of Bacteriology, September 2005, p. 6005-6018, Vol. 187, No. 17
0021-9193/05/$08.00+0     doi:10.1128/JB.187.17.6005-6018.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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