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Journal of Bacteriology, September 2005, p. 6069-6074, Vol. 187, No. 17
0021-9193/05/$08.00+0     doi:10.1128/JB.187.17.6069-6074.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

MtdC, a Novel Class of Methylene Tetrahydromethanopterin Dehydrogenases

Julia A. Vorholt,1,{dagger} Marina G. Kalyuzhnaya,2,{dagger} Christoph H. Hagemeier,3 Mary E. Lidstrom,2,4 and Ludmila Chistoserdova2*

Laboratorie des Interactions Plantes-Microorganismes, 31326 Castanet-Tolosan, France,1 Department of Chemical Engineering, University of Washington, Seattle, Washington 98195,2 Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany,3 Department of Microbiology, University of Washington, Seattle, Washington 981954

Received 5 April 2005/ Accepted 16 June 2005

Novel methylene tetrahydromethanopterin (H4MPT) dehydrogenase enzymes, named MtdC, were purified after expressing in Escherichia coli genes from, respectively, Gemmata sp. strain Wa1-1 and environmental DNA originating from unidentified microbial species. The MtdC enzymes were shown to possess high affinities for methylene-H4MPT and NADP but low affinities for methylene tetrahydrofolate or NAD. The substrate range and the kinetic properties revealed by MtdC enzymes distinguish them from the previously characterized bacterial methylene-H4MPT dehydrogenases, MtdA and MtdB. While revealing higher sequence similarity to MtdA enzymes, MtdC enzymes appear to fulfill a function homologous to the function of MtdB, as part of the H4MPT-linked pathway for formaldehyde oxidation/detoxification.

* Corresponding author. Mailing address: 231 Wilcox Hall, Box 352125, University of Washington, Seattle, WA 98195. Phone: (206) 543-6683. Fax: (206) 616-5721. E-mail: milachis{at}u.washington.edu.

{dagger} J.A.V. and M.G.K. contributed equally to this work.

Journal of Bacteriology, September 2005, p. 6069-6074, Vol. 187, No. 17
0021-9193/05/$08.00+0     doi:10.1128/JB.187.17.6069-6074.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kalyuzhnaya, M. G., Bowerman, S., Nercessian, O., Lidstrom, M. E., Chistoserdova, L. (2005). Highly Divergent Genes for Methanopterin-Linked C1 Transfer Reactions in Lake Washington, Assessed via Metagenomic Analysis and mRNA Detection. Appl. Environ. Microbiol. 71: 8846-8854 [Abstract] [Full Text]  


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