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Journal of Bacteriology, September 2005, p. 6561-6564, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6561-6564.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evolutionary and Diagnostic Implications of Intragenomic Heterogeneity in the 16S rRNA Gene in Aeromonas Strains

Alessia Morandi,1,{dagger} Olga Zhaxybayeva,2,{ddagger} J. Peter Gogarten,2 and Joerg Graf2*

Institute for Infectious Diseases, University of Berne, Berne, Switzerland,1 Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut2

Received 31 May 2005/ Accepted 7 July 2005

Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by ≤1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution when using SSU to identify aeromonads.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, University of Connecticut, 91 N. Eagleville Rd., Unit-3125, Storrs, CT 06269-3125. Phone: (860) 486-9284. Fax: (860) 486-4331. E-mail: joerg.graf{at}uconn.edu.

{dagger} Present address: Servizio Psico-Sociale, Vicolo Scottocorte 4, 6500 Bellinzona, Switzerland.

{ddagger} Present address: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada.

Journal of Bacteriology, September 2005, p. 6561-6564, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6561-6564.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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