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Journal of Bacteriology, September 2005, p. 6571-6576, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6571-6576.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Transcriptome Analysis of Pseudomonas aeruginosa Growth: Comparison of Gene Expression in Planktonic Cultures and Developing and Mature Biofilms{dagger}

Richard D. Waite,1* Anastasia Papakonstantinopoulou,1 Eddie Littler,2 and Michael A. Curtis1

MRC Molecular Pathogenesis Research Unit, Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and the London, Queen Mary School of Medicine and Dentistry, London, E1 2AT, United Kingdom,1 Medivir UK Ltd., Peterhouse Technology Park, 100 Fulbourn Road, Cambridge CB1 9PT, United Kingdom2

Received 4 February 2005/ Accepted 6 June 2005

The transcriptomes of logarithmic- and stationary-phase Pseudomonas aeruginosa planktonic cultures and static biofilms of different stages of development were compared. Developing and confluent biofilm transcriptomes were found to be related to those of logarithmic- and stationary-phase planktonic cultures, respectively. In addition, a number of novel genes were up-regulated in developing and confluent biofilms, including genes encoding putative solute transport proteins and transcriptional regulators, respectively.

* Corresponding author. Mailing address: MRC Molecular Pathogenesis Research Unit, Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and the London, Queen Mary School of Medicine and Dentistry, 4 Newark Street, London, E1 2AT, United Kingdom. Phone: 020 7882 2319. Fax: 020 7882 2181. E-mail: R.D.Waite{at}qmul.ac.uk.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, September 2005, p. 6571-6576, Vol. 187, No. 18
0021-9193/05/$08.00+0     doi:10.1128/JB.187.18.6571-6576.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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