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Journal of Bacteriology, October 2005, p. 6841-6844, Vol. 187, No. 19
0021-9193/05/$08.00+0     doi:10.1128/JB.187.19.6841-6844.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutation at the "Exit Gate" of the Salmonella Gyrase A Subunit Suppresses a Defect in the Gyrase B Subunit

Anne-Béatrice Blanc-Potard,1* Gilles Labesse,2 Nara Figueroa-Bossi,3 and Lionello Bossi3

INSERM U431, Faculté de Médecine, Avenue J. F. Kennedy, 30908 Nîmes cedex 02, France,1 Centre de Biochimie Structurale, INSERM U554-CNRS UMR5048, Université Montpellier I, 15 Av. Charles Flahault, 34060 Montpellier cedex, France,2 Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette cedex, France3

Received 7 July 2005/ Accepted 12 July 2005

In Salmonella enterica serovar Typhimurium, an S431P substitution in the B subunit of gyrase (allele gyrB651) confers resistance to nalidixic acid and causes reduced DNA superhelicity and hypersensitivity to novobiocin. Selection for novobiocin resistance allowed isolation of a mutation in the gyrA gene (allele gyrA659), a T467S substitution, which partially suppresses the supercoiling defect of gyrB651. Modeling analysis suggests that this mutation acts by destabilizing the GyrA bottom dimer interface. This is the first example of a gyrA mutation that compensates for a gyrB defect.

* Corresponding author. Mailing address: Avenir team Institut National de la Santé et de la Recherche Médicale, Unité 431, Faculté de Médecine, CS 83021, Avenue J. F. Kennedy, 30908 Nîmes Cedex 02, France. Phone: 33 4 66 02 81 47. Fax: 33 4 66 02 81 48. E-mail: ablancpotard{at}univ-montp1.fr.

Journal of Bacteriology, October 2005, p. 6841-6844, Vol. 187, No. 19
0021-9193/05/$08.00+0     doi:10.1128/JB.187.19.6841-6844.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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