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Journal of Bacteriology, January 2005, p. 449-457, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.449-457.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Error-Prone DNA Polymerase IV Is Regulated by the Heat Shock Chaperone GroE in Escherichia coli

Jill C. Layton and Patricia L. Foster*

Department of Biology, Indiana University, Bloomington, Indiana

Received 29 August 2004/ Accepted 19 October 2004

An insertion in the promoter of the operon that encodes the molecular chaperone GroE was isolated as an antimutator for stationary-phase or adaptive mutation. The groE operon consists of two genes, groES and groEL; point mutations in either gene conferred the same phenotype, reducing Lac+ adaptive mutation 10- to 20-fold. groE mutant strains had 1/10 the amount of error-prone DNA polymerase IV (Pol IV). In recG+ strains, the reduction in Pol IV was sufficient to account for their low rate of adaptive mutation, but in recG mutant strains, a deficiency of GroE had some additional effect on adaptive mutation. Pol IV is induced as part of the SOS response, but the effect of GroE on Pol IV was independent of LexA. We were unable to show that GroE interacts directly with Pol IV, suggesting that GroE may act indirectly. Together with previous results, these findings indicate that Pol IV is a component of several cellular stress responses.

* Corresponding author. Mailing address: Department of Biology, Indiana University, Jordan Hall, 1001 East Third St., Bloomington, IN 47405. Phone: (812) 855-4084. Fax: (812) 855-6705. E-mail: plfoster{at}indiana.edu.

Journal of Bacteriology, January 2005, p. 449-457, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.449-457.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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