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Journal of Bacteriology, January 2005, p. 747-751, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.747-751.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification and Complementation of a Mutation Associated with Loss of Mycoplasma pneumoniae Virulence-Specific Proteins B and C

Robert H. Waldo III, Jarrat L. Jordan,{dagger} and Duncan C. Krause*

Department of Microbiology, University of Georgia, Athens, Georgia

Received 30 August 2004/ Accepted 18 October 2004

A mutation in gene MPN142 (orf6) was identified in the Mycoplasma pneumoniae cytadherence mutant III-4. MPN142 encodes virulence-specific proteins P90 and P40 (proteins B and C, respectively). Analysis of MPN142 in a cytadhering revertant and complementation using a recombinant wild-type allele confirmed the role of this mutation in the cytadherence defect.

* Corresponding author. Mailing address: Department of Microbiology, 523 Biological Sciences Bldg., University of Georgia, Athens, GA 30602. Phone: (706) 542-2671. Fax: (706) 542-2674. E-mail: dkrause{at}uga.edu.

{dagger} Present address: Infectious Diseases Research, Centocor, Inc., Malvern, PA 19355.

Journal of Bacteriology, January 2005, p. 747-751, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.747-751.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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