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Journal of Bacteriology, January 2005, p. 758-764, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.758-764.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Structural and Genetic Characterization of Enterohemorrhagic Escherichia coli O145 O Antigen and Development of an O145 Serogroup-Specific PCR Assay{dagger}

Lu Feng,1,2,3 Sof'ya N. Senchenkova,4 Jiang Tao,1 Alexander S. Shashkov,4 Bin Liu,1 Sergei D. Shevelev,4 Peter R. Reeves,5 Jianguo Xu,6 Yuriy A. Knirel,4 and Lei Wang1,2,3*

TEDA School of Biological Sciences and Biotechnology,1 Tianjin State Laboratory of Microbial Functional Genomics, TEDA College, Nankai University,2 Tianjin Biochip Corporation, TEDA, Tianjin,3 National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China,6 N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia,4 School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia5

Received 31 May 2004/ Accepted 4 October 2004

Enterohemorrhagic Escherichia coli O145 strains are emerging as causes of hemorrhagic colitis and hemolytic uremic syndrome. In this study, we present the structure of the E. coli O145 O antigen and the sequence of its gene cluster. The O145 antigen has repeat units containing three monosaccharide residues: 2-acetamido-2-deoxy-D-glucose (GlcNAc), 2-acetamidoylamino-2,6-dideoxy-L-galactose, and N-acetylneuraminic acid. It is very closely related to Salmonella enterica serovar Touera and S. enterica subsp. arizonae O21 antigen. The E. coli O145 gene cluster is located between the JUMPStart sequence and the gnd gene and consists of 15 open reading frames. Putative genes for the synthesis of the O-antigen constituents, for sugar transferase, and for O-antigen processing were annotated based on sequence similarities and the presence of conserved regions. The putative genes located in the E. coli O145 O-antigen gene cluster accounted for all functions expected for synthesis of the structure. An E. coli O145 serogroup-specific PCR assay based on the genes wzx and wzy was also developed by screening E. coli and Shigella isolates of different serotypes.

* Corresponding author. Mailing address: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA College, 23# HongDa St., TEDA, Tianjin 300457, People's Republic of China. Phone: 86-22-66229592. Fax: 86-22-66229596. E-mail: wanglei{at}nankai.edu.cn.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, January 2005, p. 758-764, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.758-764.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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