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Journal of Bacteriology, January 2005, p. 800-804, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.800-804.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SigB-Dependent In Vitro Transcription of prfA and Some Newly Identified Genes of Listeria monocytogenes Whose Expression Is Affected by PrfA In Vivo

Marcus Rauch,{dagger} Qin Luo,{dagger} Stefanie Müller-Altrock, and Werner Goebel*

Biocenter (Microbiology), University of Würzburg, Würzburg, Germany

Received 9 August 2004/ Accepted 15 October 2004

Recent studies have identified several new genes in Listeria monocytogenes which are positively or negatively affected by PrfA and grouped into three classes (E. Milohanic et al., Mol. Microbiol. 47:1613-1625, 2003). In vitro transcription performed with promoters of some class III genes showed strict SigB-dependent but PrfA-independent transcription initiation. Transcription starting at the prfA promoter PprfA2 was also optimal with SigB-loaded RNA polymerase, suggesting a direct link between SigB- and PrfA-dependent gene expression.

* Corresponding author. Mailing address: Biocenter (Microbiology), University of Würzburg, 97074 Würzburg, Germany. Phone: 49 931 8884401. Fax: 49 931 8884402. E-mail: goebel{at}biozentrum.uni-wuerzburg.de.

{dagger} M.R. and Q.L. contributed equally to this work.

Journal of Bacteriology, January 2005, p. 800-804, Vol. 187, No. 2
0021-9193/05/$08.00+0     doi:10.1128/JB.187.2.800-804.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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