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Journal of Bacteriology, October 2005, p. 6953-6961, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.6953-6961.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nucleotide Excision Repair or Polymerase V-Mediated Lesion Bypass Can Act To Restore UV-Arrested Replication Forks in Escherichia coli

Charmain T. Courcelle,1* Jerilyn J. Belle,2 and Justin Courcelle1

Department of Biology, P.O. Box 751, Portland State University, Portland, Oregon 97207-0751,1 Department of Biological Sciences, Box GY, Mississippi State University, Mississippi State, Mississippi 397622

Received 9 May 2005/ Accepted 25 July 2005

Nucleotide excision repair and translesion DNA synthesis are two processes that operate at arrested replication forks to reduce the frequency of recombination and promote cell survival following UV-induced DNA damage. While nucleotide excision repair is generally considered to be error free, translesion synthesis can result in mutations, making it important to identify the order and conditions that determine when each process is recruited to the arrested fork. We show here that at early times following UV irradiation, the recovery of DNA synthesis occurs through nucleotide excision repair of the lesion. In the absence of repair or when the repair capacity of the cell has been exceeded, translesion synthesis by polymerase V (Pol V) allows DNA synthesis to resume and is required to protect the arrested replication fork from degradation. Pol II and Pol IV do not contribute detectably to survival, mutagenesis, or restoration of DNA synthesis, suggesting that, in vivo, these polymerases are not functionally redundant with Pol V at UV-induced lesions. We discuss a model in which cells first use DNA repair to process replication-arresting UV lesions before resorting to mutagenic pathways such as translesion DNA synthesis to bypass these impediments to replication progression.

* Corresponding author. Mailing address: Department of Biology, P.O. Box 751, Portland State University, Portland, OR 97207-0751. Phone: (503) 725-3866. Fax: (503) 725-3888. E-mail: charme{at}pdx.edu.

Journal of Bacteriology, October 2005, p. 6953-6961, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.6953-6961.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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