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Journal of Bacteriology, October 2005, p. 7146-7149, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7146-7149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Site-Directed Mutagenesis and Expression of the Soluble Form of the Family IIIa Cellulose Binding Domain from the Cellulosomal Scaffolding Protein of Clostridium cellulovorans

Koichiro Murashima,{dagger} Akihiko Kosugi,{ddagger} and Roy H. Doi*

Section of Molecular and Cellular Biology, University of California, Davis, California 95616

Received 22 April 2005/ Accepted 27 July 2005

The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant wild-type and mutant CBDs from C. cellulovorans were expressed as soluble forms. Cellulose binding tests of the mutant CBDs indicated that the planar strip residues played a major role in cellulose binding and that the anchoring residues played only a minor role.

* Corresponding author. Mailing address: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616. Phone: (530) 752-3191. Fax: (530) 752-3085. E-mail: rhdoi{at}ucdavis.edu.

{dagger} Present address: Meiji Seika Kaisha, Ltd., Sakado-shi, Saitama 350-0289, Japan.

{ddagger} Present address: Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki 305-8686, Japan.

Journal of Bacteriology, October 2005, p. 7146-7149, Vol. 187, No. 20
0021-9193/05/$08.00+0     doi:10.1128/JB.187.20.7146-7149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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