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Journal of Bacteriology, December 2005, p. 8081-8087, Vol. 187, No. 23
0021-9193/05/$08.00+0     doi:10.1128/JB.187.23.8081-8087.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Involvement of SenC in Assembly of Cytochrome c Oxidase in Rhodobacter capsulatus{dagger}

Danielle L. Swem, Lee R. Swem, Aaron Setterdahl, and Carl E. Bauer*

Department of Biology, Indiana University, Bloomington, Indiana 47405

Received 23 May 2005/ Accepted 13 September 2005

SenC, a Sco1 homolog found in the purple photosynthetic bacteria, has been implicated in affecting photosynthesis and respiratory gene expression, as well as assembly of cytochrome c oxidase. In this study, we show that SenC from Rhodobacter capsulatus is involved in the assembly of a fully functional cbb3-type cytochrome c oxidase, as revealed by decreased cytochrome c oxidase activity in a senC mutant. We also show that a putative copper-binding site in SenC is required for activity and that a SenC deletion phenotype can be rescued by the addition of exogenous copper to the growth medium. In addition, we demonstrate that a SenC mutation has an indirect effect on gene expression caused by a reduction in cytochrome c oxidase activity. A model is proposed whereby a reduction in cytochrome c oxidase activity impedes the flow of electrons through the respiratory pathway, thereby affecting the oxidation/reduction state of the ubiquinone pool, leading to alterations of photosystem and respiratory gene expression.

* Corresponding author. Mailing address: Department of Biology, Jordan Hall, Bloomington, IN 47405. Phone: (812) 855-6595. Fax: (812) 856-4178. E-mail: cbauer{at}bio.indiana.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2005, p. 8081-8087, Vol. 187, No. 23
0021-9193/05/$08.00+0     doi:10.1128/JB.187.23.8081-8087.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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