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Journal of Bacteriology, December 2005, p. 8172-8180, Vol. 187, No. 23
0021-9193/05/$08.00+0     doi:10.1128/JB.187.23.8172-8180.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Proteomic Characterization of Yersinia pestis Virulence

Brett A. Chromy,* Megan W. Choi, Gloria A. Murphy, Arlene D. Gonzales, Chris H. Corzett, Brian C. Chang, J. Patrick Fitch, and Sandra L. McCutchen-Maloney*

Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, California

Received 24 January 2005/ Accepted 30 August 2005

The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.

* Corresponding author. Mailing address: Lawrence Livermore National Laboratory, 7000 East Avenue, L-452, Livermore, CA 94550. Fax: (925) 422-2282. Phone for Brett A. Chromy: (925) 422-2454. E-mail: chromy{at}llnl.gov. Phone for Sandra L McCutchen-Maloney: (925) 423-5065. smaloney{at}llnl.gov.

Journal of Bacteriology, December 2005, p. 8172-8180, Vol. 187, No. 23
0021-9193/05/$08.00+0     doi:10.1128/JB.187.23.8172-8180.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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