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Journal of Bacteriology, December 2005, p. 8185-8190, Vol. 187, No. 23
0021-9193/05/$08.00+0     doi:10.1128/JB.187.23.8185-8190.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Pseudomonas aeruginosa Proteome during Anaerobic Growth{ddagger}

Manhong Wu,1,{dagger} Tina Guina,2,{dagger} Mitchell Brittnacher,1 Hai Nguyen,1 Jimmy Eng,3 and Samuel I. Miller1*

Departments of Medicine, Microbiology, and Genome Sciences,1 Department of Pediatrics, Division of Infectious Diseases,University of Washington,2 Fred Hutchinson Cancer Research Center, Seattle, Washington3

Received 7 September 2005/ Accepted 16 September 2005

Isotope-coded affinity tag analysis and two-dimensional gel electrophoresis followed by tandem mass spectrometry were used to identify Pseudomonas aeruginosa proteins expressed during anaerobic growth. Out of the 617 proteins identified, 158 were changed in abundance during anaerobic growth compared to during aerobic growth, including proteins whose increased expression was expected based on their role in anaerobic metabolism. These results form the basis for future analyses of alterations in bacterial protein content during growth in various environments, including the cystic fibrosis airway.

* Corresponding author. Mailing address: Departments of Medicine, Microbiology, and Genome Sciences, University of Washington, HSB K140, Box 357710, Seattle, WA 98195. Phone: (206) 616-5107. Fax: (206) 616-5109. E-mail: millersi{at}u.washington.edu.

{ddagger} Supplemental material for this article may be found at http://jb.asm.org/.

{dagger} These authors contributed equally to this work.

Journal of Bacteriology, December 2005, p. 8185-8190, Vol. 187, No. 23
0021-9193/05/$08.00+0     doi:10.1128/JB.187.23.8185-8190.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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