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Journal of Bacteriology, December 2005, p. 8526-8530, Vol. 187, No. 24
0021-9193/05/$08.00+0     doi:10.1128/JB.187.24.8526-8530.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inhibition of Bacillus subtilis scoC Expression by Multicopy senS

Eiji Kawachi, Sadanobu Abe, and Teruo Tanaka*

Department of Marine Science, School of Marine Science and Technology, Tokai University, 3-20-1 Shimizuorido, Shizuoka 424-8610, Japan

Received 27 July 2005/ Accepted 25 September 2005

The Bacillus subtilis aprE gene, which encodes the extracellular alkaline protease, is regulated by many positive and negative transcriptional regulators. SenS is one such positive regulator consisting of 65 amino acids. We found that the senS gene on a multicopy plasmid, pSEN24, caused an increase in aprE expression in strains carrying the upstream region of aprE up to –340 with respect to the transcription initiation site but not in a strain carrying the region up to –299, which is within the binding site of the negative regulator ScoC (Hpr). Epistatic analysis showed that the pSEN24 effect was lost in a scoC-deleted mutant. In accordance with these results, the scoC transcription level as assayed by a scoC-lacZ fusion and Northern analysis was greatly reduced in the cells carrying pSEN24. From these results we conclude that multicopy senS enhances aprE expression by suppressing the transcription of scoC.

* Corresponding author. Mailing address: Department of Marine Science, School of Marine Science and Technology, Tokai University, 3-20-1 Shimizuorido, Shizuoka 424-8610, Japan. Phone: 81-543-34-0411, ext. 2933. Fax: 81-543-34-9834. E-mail: teruot{at}scc.u-tokai.ac.jp.

Journal of Bacteriology, December 2005, p. 8526-8530, Vol. 187, No. 24
0021-9193/05/$08.00+0     doi:10.1128/JB.187.24.8526-8530.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Abe, S., Yasumura, A., Tanaka, T. (2009). Regulation of Bacillus subtilis aprE Expression by glnA through Inhibition of scoC and {sigma}D-Dependent degR Expression. J. Bacteriol. 191: 3050-3058 [Abstract] [Full Text]  


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