This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Endo, R. Articles by Nagata, Y. Search for Related Content PubMed PubMed Citation Articles by Endo, R. Articles by Nagata, Y.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2005, p. 847-853, Vol. 187, No. 3
0021-9193/05/$08.00+0     doi:10.1128/JB.187.3.847-853.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Genes Involved in the Downstream Degradation Pathway of -Hexachlorocyclohexane in Sphingomonas paucimobilis UT26

Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai,¹ Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata, Japan²

Received 25 August 2004/ Accepted 2 November 2004

Sphingomonas paucimobilis UT26 utilizes -hexachlorocyclohexane (-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the conversion of -HCH to maleylacetate (MA) via chlorohydroquinone (CHQ) in UT26. In this study, we identified and characterized an MA reductase gene, designated linF, that is essential for the utilization of -HCH in UT26. A gene named linEb, whose deduced product showed significant identity to LinE (53%), was located close to linF. LinE is a novel type of ring cleavage dioxygenase that catalyzes the conversion of CHQ to MA. LinEb expressed in Escherichia coli transformed CHQ and 2,6-dichlorohydroquinone to MA and 2-chloromaleylacetate, respectively. Our previous and present results indicate that UT26 (i) has two gene clusters for degradation of chlorinated aromatic compounds via hydroquinone-type intermediates and (ii) uses at least parts of both clusters for -HCH utilization.

This article has been cited by other articles:

• Endo, R., Ohtsubo, Y., Tsuda, M., Nagata, Y. (2007). Identification and Characterization of Genes Encoding a Putative ABC-Type Transporter Essential for Utilization of {gamma}-Hexachlorocyclohexane in Sphingobium japonicum UT26. J. Bacteriol. 189: 3712-3720 [Abstract] [Full Text]  
• Miyazaki, R., Sato, Y., Ito, M., Ohtsubo, Y., Nagata, Y., Tsuda, M. (2006). Complete Nucleotide Sequence of an Exogenously Isolated Plasmid, pLB1, Involved in {gamma}-Hexachlorocyclohexane Degradation. Appl. Environ. Microbiol. 72: 6923-6933 [Abstract] [Full Text]  
• Sato, Y., Monincova, M., Chaloupkova, R., Prokop, Z., Ohtsubo, Y., Minamisawa, K., Tsuda, M., Damborsky, J., Nagata, Y. (2005). Two Rhizobial Strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, Encode Haloalkane Dehalogenases with Novel Structures and Substrate Specificities. Appl. Environ. Microbiol. 71: 4372-4379 [Abstract] [Full Text]